Korean Journal of Plant Tissue Culture (식물조직배양학회지)

The Korean Society of Plant Biotechnology (한국식물생명공학회)
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Trehalose Metabolism: Gate to Stress Signaling and Seed Development in Plant\ulcorner

  • Chung, H-J (Kumho Life & Environmental Science Laboratory) ;
  • Kim, Y-S (Kumho Life & Environmental Science Laboratory) ;
  • Lee, E-J (Kumho Life & Environmental Science Laboratory) ;
  • Kim, J-S (Kumho Life & Environmental Science Laboratory) ;
  • Shin, Y-M (Kumho Life & Environmental Science Laboratory) ;
  • Cho, I-S (Kumho Life & Environmental Science Laboratory) ;
  • Jin, H-O (Kumho Life & Environmental Science Laboratory) ;
  • Cho, J-W (Kumho Life & Environmental Science Laboratory) ;
  • Chung, C-H (Kumho Life & Environmental Science Laboratory)
  • Published : 2000.09.01
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Abstract

The disaccharide trehalose ($\alpha$-D-glucopyranosyl-$\alpha$-D-glucopyranoside) is found in variety of organ-isms that are able to withstand almost complete desiccation. In order to identify the function of trehalose in plants, we isolated Arabidopsis trehalase (AtTRE) gene that encodes the enzyme able to hydrolyze trehalose to glucose, and trehalose-6-phosphate synthase isolog, TPS3 gene by RT-PCR. The AtTRE had the substrate specificity to hydrolyze only trehalose, and a broad pH range of enzyme activity. The AtTRE promoter/GUS reporter gene was expressed in cotyledons, mature leaf tissues including guard cells, and developing siliques. The GUS expression driven by AtTPS3 promoter was significant in root tissues, and the level of GUS activity was much higher than that of the pBll 21 control seedlings. The knockout of AtTPS3 gene in Arabidopsis resulted in the retarded root development, whereas the overexpression of AtTPS3 increased the root elongation in the presence of sucrose in MS medium. Possible functions of AtTRE and AtTPS3 in plant will be discussed. In addition, ectopic expression of yeast TPS1 driven by the inducible promoters in tobacco and potato conferred the plants on the drought and freezing tolerances.

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References

  1. Plant Physiol v.119 Purification of the trehalse GMTRE1 from soybean nodules and cloning of its cDNA. GMTRE1 is expression at a low level in multiple tissues Aeschbacher RA;Muller J;boller T;Wiemken A
  2. J. Biol. Chem. v.273 Composition and functional analysis of the Saccharomyces cerevisiae trehalose synthase complex Bell W;Sun W;Hohmann S;Wera S;Reinders A;Virgilio CD;Wiemken A;Thevelein JM
  3. Plant J. v.13 Isolation and molecular characterization of the Arabidopsis TPS1 gene, encoding trehalose-6-phosphate synthase Blazquez MA;Santo E;Flores C-L;Martinez-Zapater JM;Salinas J;Gancedo C
  4. Trends in Plant Sciences v.4 trehalose metabolism in plants Goddijn OJM;Kun KV
  5. Plant J. v.14 Sensing trehalose biosynthesis in plants Goddijn OJM;Smeekens S
  6. Plant Physiol. v.113 Inhibition of trehalase activity enhances trehalose accumulation in transgenic plants Goddijn OJM;Verwoerd TC;Voogd E;Krutwagen RWHH;Graaf PTHM;Poels J;Kun KV;Ponstein AS;Damm B;Pen J
  7. FEMS Microbiol. Letters v.154 Trehalases and trehalose hydrolysis in fungi Jorge JA;Polizeli MLTM;Thevelein JM;Ternzi HF
  8. Planta v.197 Effects of validamycin A, a potent trehalase inhibitor, and phytohormones on trehalose metabolism in roots and root nodules of soybean and cowpea Muller J;Boller T;Wiemke A
  9. Plant Science v.147 Trehalose metabolism in sugar sensing and plant development Muller J;Wiemke A;aeschbacher R
  10. Plant Science v.149 Differential synthesis of sucrose and trehalose in Euglena gracilis cells during growth and salt stress Popchia AC;Fio DF;Salerno GL
  11. Mol. Microbiol. v.24 Structural analysis of the subunits of the trehalose-6-phosphate synthase/phosphatase complex in Saccharomyces cerevisiae and their function during heat shock Reinders A;Burchert N;Hohmann S;thevelein JM;Boller T;Wiemken A;Virgilio CD
  12. Planta v.201 Expression of the yeast trealose-6-phosphate synthase gene in transgenic tobacco plants: pleiotropic phenotypes include drought tolerance Romero C;Belles JM;Vaya JL;Serrano R;Culianez-Macia FA
  13. Trends in Biotechnol. v.16 Thermotolerance in Saccharomyces cerevisiae: the Yin and Yang of trehalose Singer MA;Lindquist S
  14. Plant J. v.13 Threhalose-6-phosphate phosphatases from Arabidopsis thaliana: identification by functional complementation of the yeast tps2 mutant Vogel G;Aeschbacher RA;Muller J;Boller T;Wiemken A
  15. Mol. Cells v.10 Genetic engineering of drought resistant potato plants by introduction of the trehalose-6-phosphate synthase (TPS1) gene from Saccharomyces cerevisiae Yeo ET;Kwon H-B;Han S-E;lee J-T;Ryu J-C;Byun M-O
  16. Plant Physiol. v.119 A Selaginella lepidophylla trehalose-6-phosphate synthasecomplements growth and stress-tolerance defects in a yeast tps1 mutant Zentella R;Mascorro-Gallardo JO;Dijck PV;Folch-Mallol J;Bonini B;Vaeck CV;Gaxila R;Covarrubias AA;Nieto-Sotelo J;Thevelein JM;Iturriaga G