Korean Journal of Environmental Biology (환경생물)

Korean Society of Environmental Biology (한국환경생물학회)
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Is Cadmium Pretreatment-Induced Protection against Cadmium Lethality to Mice Related to the Hepatic Glutathione Contents\ulcorner

카드뮴 전처리에 의한 생쥐의 카드뮴 치사 완화효과와 간 glutathione 함량과의 상관성

  • Published : 2000.03.01
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Abstract

Which sublethal cadmium pretreatment may prevent from lethal cadmium's killing mice and which cadmium pretreatment-induced protection against cadmium lethality to mice may be related with their hepatic glutathione contents were investigated. When cadmium chloride was subcutaneously injected to mice (ICR strain) at various doses, all mice died, which treated with cadmium at dose of 300 $\mu$moles/kg or more, and none died, which treated with cadmium at dose of 80 $\mu$moles/kg or less. Subcutaneous pretreatment of sublethal cadmium decreased sacrifice of mice which subsequently injected with lethal cadmium, with most effectiveness at pretreatment dose of cadmium of 40 $\mu$moles/kg b.w. and at 48 hours of interval between sublethal cadmium pretreatment and lethal cadmium treatment. Even if a great part of the cadmium-pretreated mice were sacrificed while treated with lethal cadmium, they survived longer than the non-pretreated mice. Sublethal cadmium pretreatment (40 $\mu$moles/kg b.w.) 48 hours before lethal cadmium treatment to mice didn't decrease hepatic glutathione contents of the survived mice, while decreases in the glutathione in livers were observed in the mice just after died. These results indicate that sublethal cadmium pretreatment-induced protection against cadmium lethality to mice may be related to their hepatic glutathione contents. [Cd pretreatment, Cd lethality, Hepatic glutathione contents].

생쥐에서 카드뮴 전 처리에 의하여 카드뮴의 치사독성이 완화되는지를 조사하고 치사완화 효과와 간 조직내의 glutathione(GSH)함량이 상관성이 있는지를 조사하였다. 치사량의 카드뮴을 주사하기 전에 치사량 이하의 카드뮴을 주사하면 치사량의 카드뮴에 의한 치사작용이 완화되는 효과가 나타났으며 카드뮴의 전 처리의 경과시간은 48시간의 경우에, 전 처리량은 체중 kg당 40$\mu$moles을 주사한 경우에 효과가 가장 좋았다. 카드뮴을 전 처리한 생쥐는 카드뮴에 의해 치사되는 경우에도 그 생존기간이 최대 72시간까지 연장되었다. 치사량의 카드뮴을 주사한 경우에 카드뮴 전 처리에 의하여 생존한 개체들은 간조직내의 glutathione함량은 대조군에 비하여 별다른 차이가 없었으나 치사한 개체들은 glutathione 함량이 감소하였고 또한 전처리를 하지 않은 실험군은 치사량의 카드뮴에 의하여 glutathione 함량이 감소하였다. 이러한 실험결과는 치사량 이하의 카드뮴을 생쥐에 전 처리하면 치사작용을 완화하는 인자들이 유도되어 후속 주사하는 치사량의 카드뮴에 의한 치사작용을 완화하고, 간 조직내의 glutathione이 카드뮴 치사작용에 대한 방어인자가 될 수 있음을 암시한다.

Keywords

References

  1. 생명과학(삼육대학교부설 생명과학연구소 논문집) v.1 흰쥐에서 카드뮴 전처리에 의한 치사방지효과와 간과 뇌의 glutathione과 glucose 수준과의 상관성 부문종;김선희
  2. 생명과학(삼육대학교부설 생명과학연구소 논문집) v.3 생쥐 간의 glutathione과 glucose 함량에 미치는 비소의 영향 부문종
  3. 생명과학(삼육대학교부설 생명과학연구소 논문집) v.4 비소에 으한 생쥐의 치사독성과 간 글리코겐 함량과의 상관성 부문종
  4. Toxicol. Pathol. v.24 Enhanced arsenite-induced hepatic morphological and biochemical changes in phenobarbital-pretreated rats Albores A;ME Cebrian;GG Garcia-Vargas;JC Connelly;SC Price;RH Hinton;PH Bach;JW Bridges
  5. Biol. Trace Elem. Res. v.52 Cadmium-induced excretion of urinary lipid metabolites, DNA damage, glutathione depletion, and hepatic lipid peroxidation in Sprague-Dawley rats Bagchi D;M Bagchi;EA Hassoun;SJ Stohs
  6. Life Sci. v.56 Cadmium-induced DNA fragmentation is inhibitable by zinc porcine kidney LLC-PK1 cells Ishido M;ST Homma;PS Leung;C Tohyama
  7. Arch. Toxicol. v.69 Influence of glucose on the toxicity of oxophenylarsine in MDCK cells Liebl B;H Muckter;E Doklea;FX Reichl;B Fichtl;W Forth
  8. Fundam. Appl. Toxicol. v.27 Reversal of oxophenylarsine-induced inhibition of glucose uptake in MDCK cells Liebl B;H Muckter;E Doklea;B Fichtl;W Forth
  9. J. Pharmacol. Exp. Therap. v.232 High-affinity lead binding proteins in rat kidney cytosol mediate cell-free nuclear translocation of lead Mistry P;GW Lucier;BA Fowler
  10. Arch. Toxicol. v.64 Effect of glucose in mice after acute experimental poisoning with arsenic trioxide(As₂O₃) Reichl FX;L Szinicz;H Kreppel;B Fichtl;W Forth
  11. Vet. Hum. Toxicol. v.33 Effects of glucose treatment on carbohydrate content in various organs on mice after acute As₂O₃ poisoning Reichl FX;H Kreppel;L Szinicz;B Fichtl;W Forth
  12. Toxicol. Appl. Pharmacol. v.141 Induction of hsp70 in HepG2 cells in response to hepatotoxicants Salminen WFJr;R Voellmy;SM Roberts
  13. Methods in Enzymology v.113 Glutathione biosynthesis Selig GF;A Meister;Meister A(ed.)
  14. FASEB J. v.1 Glutathione, a first line defense against cadmium Singhal RK;ME Anderson;A Meister
  15. Jpn. J. Med. Sci. Biol. v.49 Realtionship between glutathione and DL alpha-lipoic acid against cadmium-induced hepatotoxicity Sumathi R;G Baskaran;P Varalakshmi
  16. Arch. Toxicol. v.61 Effect of As₂O₃ on gluconeogenesis Szinicz L;W Forth
  17. FEBS Lett. v.245 The human 32-kDa stress protein induced by exposure to arsenite and cadmium ions is heme oxygenase Taketani S;H Kohno;T Yoshinaga;R Tokunaga
  18. Eur. J. Biochem. v.165 Isolation of biologically active low-molecular mass, chromium binding compounds from rabbit liver Yamamoto A;O Wada;T Ono
  19. Biochem. Biophys. Res. Commun. v.202 A proteolytic activity enhanced by arsenite in chinese hamster ovary cells : possible involvement in arsenite-induced cell killing Yih LH;TC Lee
  20. Toxicol. Lett. v.87 Hepatocytes from metallothionein-Ⅰ and Ⅱ knock-out mice are sensitive to cadmium- and tert-butylhydroperoxide-in-duced cytotoxicity Zheng H;J Liu;Y Liu;DD Klaassen