Effect of Soil Moisture and Texture on Saikosaponins Content and Antioxidative Enzyme Activities in Bupleurum falcatum L.

재배토양의 수분 및 토성이 시호의 생육상황 및 항산화효소 활성에 미치는 영향

  • 정형진 (안동대학교 생명자원과학부) ;
  • 신동현 (경북대학교 농과대학 농학과) ;
  • 이인중 (경북대학교 농과대학 농학과) ;
  • 권순태 (안동대학교 생명자원과학부) ;
  • 임종국 (안동대학교 생명자원과학부) ;
  • 유정민 (안동대학교 생명자원과학부) ;
  • 정규영 (안동대학교 생명자원과학부) ;
  • 김길웅 (경북대학교 농과대학 농학과)
  • Published : 2000.08.01

Abstract

To study the effects of soil moisture and texture on characteristics of growth, content of saikosaponins and activity of antioxidative enzymes in Bupleurum falcatum L., content of saikosaponins(a, c and d) and activities of superoxide dismutase(SOD) and peroxidase(POD) were investigated with two Bupleurum genotypes(Jangsoo and Samdo). Two Bupleurum genotypes were grown under different soil moisture(deficit, normal, surplus) and soil texture(sandy, sandy loam, loam) conditions. Among the tested soil conditions, dry weight accumulation rate of both cultivars could be ranked in the order surplus > normal > deficit soil for soil moisture and sandy > sandy loam > loam for soil texture. Under the surplus soil condition, growth retardation of Samdo cultivar was more severer than that of Jangsoo. Furthermore, content of saikosaponin a, d, and c also could be ranked in the order deficit > normal > surplus and sandy > sandy loam > loam for soil moisture and texture, respectively. Although both Jangsoo and Samdo cultivars grown under water deficit condition showed the highest POD and SOD activity, in general POD and SOD activity in both shoot and root was remarkably high in Jangsoo cultivar compared with Samdo. Saikosaponin content of root was positively correlated with POD and SOD. However, shoot and root length were negatively correlated with POD.

토성 및 토양수분에 따른 시호근의 생육특성과 saikosaponin함량 및 항산화효소 활성 변화를 조사해 본 결과, 수분과다 토양에서 는 삼도시 호는 장수시호보다 생육 억제정도가 매우 높았고, 건물중은 과습, 적습, 과건 재배 순으로 높았고, 수분조건에 따른 토성별로는 과건은 식양토, 사양토, 사토 순으로, 적습과 과습시는 사토, 사양토, 식양토 순으로 높았다. 토양수분조건별 saikosapon a, d, c함량은 과건, 적습, 과습 순으로 높았고, 토성간에는 사토, 사양토, 식양토 순으로 높았다. POD 활성은 지상부는 장수시호가 삼도에 비하여 매우 높았고, 토양 수분함량간에는 장수는 과건, 과습, 적습 조건 순으로 높았으나, 삼도시 호는 과건, 적습, 과습 순으로 높았다. SOD활성은 지상부와 지하부 공히 장수시호가 높은 경향이나, 수분조건별로는 과건, 과습, 적습순으로 높았으며, 건조상태의 경우 두 품종 모두 사토에서 높았다. 시호에서 뿌리의 Saikosaponin함량과 POD 및 SOD 함량과는 정의상관을 나타내는 경향이었고 줄기 및 뿌리길이와 POD함량과는 부의 상관을 나타내었다.

Keywords

References

  1. Antioxidants in higher plants. Alscher RG;Hes JL
  2. Physiol Plant. v.85 Ascorbate peroxidase-a hydrogen peoxide-sacvenging enzyme in plants. Asada K
  3. CRC Critical Reviews in Biochemistry. v.22 Aspects of the structure, function and applications of superoxide dismutase. Bannister JV;Bannister WH;Rotilio G
  4. Anal Biochem v.44 Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels. Beauchamp C;Freidovich I
  5. Anal Biochem v.72 A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye binding. Bradford MM
  6. 第 5 回 藥用植物栽培技術フオ-ラアム 講演旨集 西孝三郞
  7. Agriculture chemistry and biotechnology. v.38 Biochemical change in Brassica seedlings due to cold teratment. Ham, M. Hee.;W. C. Park;K. B. Park
  8. Chem. Pharm. Bull. v.38 no.2 Studies on the cultivation of Bupleurum falcatum L.(I), Effects of cultivation condition on the root growth and saponin contents. Hosoda, K.;M. Noguchi
  9. Abstract in The Koream Society of Medocal Crop Science Distribution of saikosapinins and their variation due to plant growth characters in bupleurum falcatum L. Kim, K. S.;N. S. Seong;Y. H. Chae;S. T. Lee;Y. A. Chae
  10. Kor. J. Crop Sci. v.41 Medicainal componetnts in Bupleurum species. Kim, K. S.;S. T. Lee;Y, A, Chae
  11. J. Bol. Chem. v.244 Superoxide disutase. An enzymic function for erythrocuprein (Hemocuprein). McCord JM;Fridovich I
  12. Natural Medicines v.49 no.2 Physiological response and improvement of tolerance to environmental stress in Bupleurum falcatum L.(I) Effects of dry stress on growth and saikosaponin comtent of one-year-old, rosette plant. Minami, M.;M. Sugion;Hata;C. Hasegawa; K Ashida;K. Ogaki
  13. Physiol Plant v.15 A revised medium for rapid growth and bioassay with tobacco tissue cultere. Murashige T;Skoog F
  14. J. Pharmagon. v.21 no.1 On the comparative cultivation of Bupleurum falcatum L. from Miyazaki. Jap. Ohashi, H;T. Kuribayashi
  15. SOD and active oxygen modulators:pharmacology and clinical trials. Oyanagui Y
  16. Jap. J. Pharmagon. v.27 no.1 On the trial selection of annual crop in Bupleurum falcatum (Preliminary report). Ohashi, H;T. Kuribayashi
  17. jap. J. Pharmagon v.31 no.2 Studies on the cultivation of Bupleurum falcatum L. Ostuka, H.;S. Kobayashi;S. Shibata
  18. Korean journal of medical crop science v.3 no.3 Plug sedling production using tissue cultue regenerants and their seeds in Bupleurum falcautum L. Park, C. H.;C. Y. Yu;J. S. Seo;K. S. Kim;S. U. Park;S. D. Ahn;B. H. Chang
  19. Res Rept. RDA> Korea. v.34 no.1 Comparative saikosaponin determination due to cultivars and root ages in Bupleurum falcatum L. Park, Y. J.;H. S. Suh;S. K. Lee;J. W. Shim
  20. Kor. J. Pharmacon. v.21 no.3 Saikosaponin contents in Bupleurum flcatum root produced by tissue culture. Jo, P. H.;R. S. Seong;H. H. Bae;W. Y. Soh;D. Y. Cho
  21. Proc. Natl. Acad. Sci. v.87 Characterization of superoxide dismutase cDNAs from plants obtained by genetic complementation in Escherichia coli. Win van Camp;Chirs Bowler;Raimundo Villarroel(ed.);WT Tsang;Marc Van Montagu;Dirk Inze