Abstract
The experiments were carried out to investigate the antimicrobial activity and the content of isoflavonoids each fraction of the methanol extract from Korean arrowroots. The strongest antimicrobical fraction of the methanol extract was the etylacetate fraction and the other fraction was not detected antimicrobial activity. The yield of the ethylacetate fractation was 7~9% of the methanol extract of Korean arrowroot TLC was carried out using silical gel plate and CH$_2$C1$_2$- $CH_3$OH(7 : 3) and puerarin, daizin were detected by spraying 10% H$_2$SO$_4$ and Rf was 0.60 and 0.69, respectively. Puerarin, daidzin and daidzein contents were determined of the ethylacetate fraction of the sample Puerarin and daidzin contents were abundant in the samples of November, while daidzein content was abundant in the sample of Februery.
칡뿌리를 이용한 건강식품의 개발 가능성을 탐색하고자 항균활성과 isoflavonoid 화합물을 분석한 결과는 다음과 같다. 항균활성은 칡뿌리의 methanol 추출물의 용매분획물중 에틸아세테이트 분획물에서 나타났으며 암칡보다는 숫칡에서 활성이 높고 그람 음성균에서 활성이 크게 나타났다. 그리고 ethyl-acetate분획물은 7~9% 수율로 얻어졌고 silical gel TLC에서 CH$_2$Cl$_2$ : $CH_3$OH=7 : 3 전개용매로 puerarin은 Rf치 0.60 daidzin은 Rf치 0.69를 나타내었으며 isoflavonoid중 purearin의 함량이 가장 많고 daidzin 그리고 daidzein은 소량 함유된 것으로 나타났으며 이들의 함량은 숫칡보다 암칡에서 높게 나타났다.
