Plant Resources
- Volume 3 Issue 3
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- Pages.173-178
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- 2000
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- 1226-8771(pISSN)
Differentiation and Detection of Phytoplasma using PCR from Diseased Plant in Korea
- Lee, Kui-Jae (Faculty of Bioresources Science, College of Agricultire, Chonbuk national Unversity)
- Published : 2000.12.01
Abstract
This test checked jujube witches'-broom disease, sumac witches'-broom disease, paulonia witches'- broom disease, and mulberry dwarf disease whether or not they were infected by phytoplasma, using universal and specific primers. Upon treatment of DNA amplified by PCR of phytoplasma with Alu I , Hpa II and Sat I restricted enzymes, distinction of phytoplasmas was possible. Particularly, phytoplasma of each host was distinguishable by treatment of Hpa II restricted enzyme. Meanwhile, analysis of restricted enzymes of jujube witches'-broom disease showed a higher infectivity of phytoplasmas of two origins. There were a lot of relations between jujube witches'-broom disease and sumac witches'-broom disease, and between paulonia witches'-broom disease and mulberry dwarf disease.