반응표면분석법을 이용한 인삼 Root 액체배양조건의 최적화

Optimization of Submerged Culture Conditions for the Production of Ginseng Root Using Response Surface Method

식물조직배양기술을 이용하여 인삼 root를 생산하고자, 식물생장조절물질로 유도된 인삼 root를 사용하여 최적액체배양조건을 RSM을 이용하여 조사하였다. 최적액체배양조건을 배지의 pH, sucrose 농도, nitrogen 농도, phosphate 농도의 3 level-4factor의 fractional factorial design에 의하여 조사한 결과, 인삼 root의 생장율은 최저 1.00g에서 최고 2.33g까지 나타났다. 다중회기분석으로 구한 model식을 가지고 등고분석과 3차원분석을 수행한 후 독립변수의 최저 또는 최고수준에서 조속변수가 최대치를 나타내지 않는 배지의 pH와 sucrose농도의 변수에 대하여 model식을 편미분한 결과 인삼 root의 최적액체배양조건은 pH5.6, sucrose 3.8%, nitrogen 50mg/L, phosphate 80.7mg/L로 예측되었다. 이렇게 결정된최적조건값들을 model식에 대입하여 얻은 예상치는 2.36g 이었다.

To develop the production of ginseng root using plant tissue culture technology, submerged culture conditions were optimized by means of the fractional factorial design with 4 factors and 3 levels by a RSM computer program. The ginseng (Panax ginseng C. A. Meyer) roots induced by plant growth regulators were cultured on SH medium and the effects of various pH of medium, sucrose concentration, nitrogen concentration and phosphate concentration on fresh weight of the ginseng root were investigated. The fresh weight of ginseng root increased with a decrease in nitrogen concentration and fresh weight of ginseng root varied from 1.00 to 2.33g under various conditions. The optimum pH of medium and sucrose concentration determined by a partial differentiation of the model equation, nitrogen and phosphate concentration were pH 5.6, sucrose 3.8%, nitrogen 50 mg/L and phosphate 80.7 mg/L, respectively. Under these conditions, the predicted growth of ginseng root was estimated to be 2.36g.