Journal of Microbiology

  • 제38권1호
  • /
  • Pages.18-23
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  • 2000
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  • 1225-8873(pISSN)
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  • 1976-3794(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Isolation of the Regulator Gene Responsible for Overproduction of Catalase A in $H_2O$$_2$-resistant Mutant of Streptomyces coelicolor

  • Hahn, Ji-Sook (Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University) ;
  • Oh, So-Young (Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University) ;
  • Keith F. Chater (Department of Genetics, John Innes Centre) ;
  • Roe, Jung-Hye (Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University)
  • 발행 : 2000.03.01
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초록

Streptomyces coelicolor produces three kinds of catalases to cope with oxidative stress and to allow normal differentiation. Catalase A is the major vegetative catalase which functions in removing hydrogen peroxide generated during the process of aerobic metabolism. To understand the regulatory mechanism of response against oxidative stress, hydrogen peroxide-resistant mutant (HR4O) was isolated from S. coelicolor J1501 following UV mutagenesis. The mutant overproduced catalase A more than 50-fo1d compared with the wild type. The mutation locus catRI was mapped closed to the mthB2 locus by genetic crossings. An ordered cosmid library of S. coelicolor encompassing the mthB2 locus was used to isolate the regulator gene (catR) which represses catalase overproduction when introduced into HR4O. A candidate catR gene was found to encode a Fur-like protein of 138 amino acids (15319 Da).

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