Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Isolation of the Regulator Gene Responsible for Overproduction of Catalase A in $H_2O$$_2$-resistant Mutant of Streptomyces coelicolor

  • Hahn, Ji-Sook (Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University) ;
  • Oh, So-Young (Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University) ;
  • Keith F. Chater (Department of Genetics, John Innes Centre) ;
  • Roe, Jung-Hye (Department of Microbiology, College of Natural Sciences, and Research Center for Molecular Microbiology, Seoul National University)
  • Published : 2000.03.01
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Abstract

Streptomyces coelicolor produces three kinds of catalases to cope with oxidative stress and to allow normal differentiation. Catalase A is the major vegetative catalase which functions in removing hydrogen peroxide generated during the process of aerobic metabolism. To understand the regulatory mechanism of response against oxidative stress, hydrogen peroxide-resistant mutant (HR4O) was isolated from S. coelicolor J1501 following UV mutagenesis. The mutant overproduced catalase A more than 50-fo1d compared with the wild type. The mutation locus catRI was mapped closed to the mthB2 locus by genetic crossings. An ordered cosmid library of S. coelicolor encompassing the mthB2 locus was used to isolate the regulator gene (catR) which represses catalase overproduction when introduced into HR4O. A candidate catR gene was found to encode a Fur-like protein of 138 amino acids (15319 Da).

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References

  1. Micrbiol. Rev v.51 Molecular mechanism of regulation of siderophore-mediated iron assimilation Bagg, A.;J.B. Neilands
  2. Gene v.116 Plasmid cloning vectors for the conjugal trasfer of DNA from Escherichia coli to Streptomyces spp. Bierman, M.;R. Logan;K. O'Brien;E.T. Seno;R.N. Rao;B.E. Schoner
  3. Mol. Microbiol. v.29 Bacillus subtilis contains multiple Fur homologues: identificaion of he iron uptake (Fur) and peroxide regulon (PerR) repressors Bsat, N.;A. Herbig;L. Casillas-Martinez;P. Setlow;J.D. Helmann
  4. Proc. Natl. Acad. Sci. USA. 92 Coordinate regulation of Bacillus subtilis peroxide stress genes by hydrogen eroxide and metal ions Chen, L.;L. Keramati;J.D. Helmann
  5. Mol. Microbiol v.35 A developmentally regulated catalase required for proper differentiation and osmoprotection of Streptomyces coelicolor Cho, Y.-H.;E.-J. Lee;J.-H. Roe
  6. J. Bacteriol v.179 Isolation and expression of the catA gene endocing the major vegetative catalase in Streptomyces coelicolor Muler Cho, Y.-H.;J.-H. Roe
  7. Positive control of a regulon for defenses against oxidative stress and some heat-shock proteins in Salmonela typhimurium v.Cell 41 Christman, M.F.;R.W. Mrgan;F.S. Jacobson;B.N. Ames
  8. Kor. J. Microbiol. (currently J. Micobiol.) v.31 Profile anlaysis of proteins related with hydrogen peroxide response in Streptomyes coelicolor (Muller) Chung, H.-J.;J.-H. Roe
  9. Microbiology v.142 Cloing, sequencing and disruption of a bromoperoxidase-catalase gene in Streptomyces venezuelae: evidence that it is not required for chlorination in chloramphenicol biosynthesis Facey, S.J.;F. Gross;L.C. Vining;K. Yaung;K.H. van Pee
  10. Microbiol. Rev. v.55 Oxidative stress responses in Escherichia coli and Salmonella typhimurium Farr, S.B.;T. Kogoma
  11. Redos signal transduction: mutations shifting [2Fe-2S] centers of the SoxR sensor--regulator to the oxidized form v.Cell 88 Hidalgo, E.;H. Ding;B. Demple
  12. Genetic manipulation of Streptomyces: a laboratory manual Hopwood, D.A.;M.J. Bibb;K.F. Chater;T. Kieser;C.J. Bruton;H.M. Kieser;D.J. Lydiate;C.P. Smith;J.M. Ward;H. Schrempf
  13. J. Gen. Microbiol v.139 The induction of oxidative enzymes in Streptomyces coelicolor upon hydrogen peroxide tretment Lee, J.-S.;Y.-C. Hah;J.-H. Roe
  14. Gene 111 Analysis of Streptomyces avermitilis genes required for avermectin biosynthesis utilizing a novel integration vector MacNeil, D.J.;K.M. Gewain;C.L. Ruby;G. Dezeny, P.H.;Gibbons;T. MacNeil
  15. J. Bacteriol v.171 Intergeneric conjugation between Escherichia coli and Streptomyces species Mazodier, P.;R. Petter;C. Thompson
  16. J. Gen. Microbiol v.96 A morphological and genetic mapping study of bald colony mutats of Streptomyces coelicolor Merrick, M.J.
  17. Mol. Microbiol v.21 A set of ordered cosmids and a detailed genetic and physical map for the 8 Mb Streptomyces coelicolor A3(2) chromosome Redenbach, M.;H.M. Kieser;D. Denapaite;A. Eichner;J. Cullum;H. Kinashi;D.A. Hopwood
  18. Curr. Opin. Microbiol v.2 Oxidative stress Storz, G.;J.A. Imlay
  19. Science 279 Activation of the OxyR transcription factor by reversible disulfide bond formation Zheng, M.;F. Aslund;G. Storz