Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Differentiation of Salmonella typhimurium from Gram-negative Intestinal Microbes by Randomly Amplified Polymorphic DNA (RAPD) Fingerprinting

  • Jin, Un-Ho (Department of Biochemistry, Molecular Biology and Pathology, College of Oriental Medicine) ;
  • Chung, Tae-Wook (Department of Biochemistry, Molecular Biology and Pathology, College of Oriental Medicine) ;
  • Kim, June-Ki (Department of Biochemistry, Molecular Biology and Pathology, College of Oriental Medicine) ;
  • Nam, Kyung-Soo (Department of Pharmacology, College of Medicine, Dongguk University) ;
  • Ha, Sang-Do (Department of Industry and Technology, Korea Health industry Development Institute) ;
  • Kim, Cheorl-Ho (Department of Biochemistry, Molecular Biology and Pathology, College of Oriental Medicine)
  • Published : 2000.03.01
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Abstract

In order to rapidly identify and differentiate Salmonella typhimurium from the intestinal gram-negative bacteria, randomly amplified polymorphic DNA (RAPD) fingerprinting of Salmonella typhimurium was carried out using random primers designated OPA-13 (5'-CAGCACCCAC-3'), OPB-10 (5'-CGTCTGGGAC-3'), OPB-18 (5'-CCACAGCAGT-3'), and OPJ-10 (5'-AAGCCCGAGG-3'), and its patterns compared with 6 representive intestinal, gram-negative bacterial strains, Vibrio parahaemolyticus, V. vulnificus, Enterobacter cloacae, Escherichia coli O157:H7, Pseudomonas aeruginosa, and Proteus sp., which are often found in foods. S. typhimurium had unique and distinct fingerprinting patterns. RAPD fingerprinting is thus concluded to be a rapid and sensitive method for the identification of S. typhimurium compared to conventional culturing procedures or immunoassays.

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