Chemopreventive Potential of Angelicae gigantis Radix Aqua-acupuncture Solution

당귀 약침액의 암예방 효과

  • 김영기 (동국대학교 한의과대학) ;
  • 조경희 (동국대학교 한의과대학) ;
  • 손윤희 (의과대학 및 난치병한양방치료연구센터) ;
  • 최혜경 (동국대학교 한의과대학) ;
  • 김소연 (영남대학교 자연자원대학) ;
  • 임종국 (동국대학교 한의과대학) ;
  • 남경수 (의과대학 및 난치병한양방치료연구센터)
  • Published : 2000.06.01

Abstract

Angelicae gigantis Radix aqua-acupuncture solution (AGRAS) and Angelicae gigantis Radix water-extracted solution (AGRWS) were prepared and tested for their organ toxicities and chemopreventive potentials. The organ-toxicity of AGRAS to male ICR mice was studied by the measurements of glutamic oxaloacetic transaminase (GOT), glutamic pyruvate transaminase (GPT), lactate dehydrogenase (LDH) and alkaline phosphatase (ALP-s) activities after injection of AGRAS for 7 days. The activities of GOT GPT and LDH were decreased, but the activity of ALP-s was not changed with AGRAS. When AGRAS was administered once daily for 10 days before the tumor implantation, AGRAS exerted antitumor activity by inhibiting the growth of Ehrich ascites tumor cells (EATC) in viva. The inductions of quinone reductase (QR), glutathione (GSH) and glutathione S-transferase (GST) and inhibition of polyamine metabolism were tested for the chemopreventive potentials of AGRAS and AGRWS. AGRAS was potent inducer of QR activity in murine hepatoma Hepalclc7 cells. In cultured rat Ac2F cells, AGRAS was also significantly induced QR activity GSH levels were increased about 1.3 fold with AGRAS. In addition the activity of GST was increased about 2.5 fold with AGRAS at the concentration of $0.1{\;}{\times}{\;}$. The effects of AGRAS and AGRWS were tested on the growth of Acanthamoeba castellanii. Proliferation of Acanthamoeba castellanii in a broth medium was inhibited by AGRAS and AGRWS at the concentration of $1{\;}{\times}{\;}and{\;}5{\;}{\times}{\;}$, respectively: These results suggest that AGRAS has chemopreventive potential by inducing QR activity increasing GSH and GST levels and inhibition of polyamine metabolism.

Keywords

References

  1. Cancer Res v.54 Screening of potential chemopreventive agents using biochemical markers of carcinogenesis Sharma. S.;Stutzman. J.D.;Kelloff. G.J.;Steele. V.E.
  2. Cancer Res v.45 Chemoprevention of cancer Wattenberg. L.W.
  3. Cancer Biology and Therapeutics Talalay. P.;DeLong. M.J.;Prochaska. H.J.;Cory. J.G.(ed.);Szentivani. A.(ed.)
  4. Adv. Cancer Res v.29 The role of glutathione and gluatathione-S-transferase in mercapturic acid biosynthesis Boyland. E.;Chasseud. C.F.
  5. Jpn. J. Pharmacol. v.37 Inhibition of teleocidincaused epidermal ornithine decarboxylase induction by phospholipase A₂, cyclooxygenase- and lipoxy genase-inhibitors Nakadate. T.;Aizu. E.;Yamamoto. S.;Fujiki. H.;Sugimura. T.;Kato. R.
  6. Science v.227 Proxidant states and tumor promotion Curutti. P.A.
  7. 李草學 李尙仁
  8. 神農本草經 吳普等
  9. 奇蹟의 藥鍼療法 金廷彦
  10. 經絡 南相干
  11. 中藥藥理與應用 王浴生
  12. 本草綱目 李時珍
  13. 大韓鍼灸學會誌 v.13 當歸 藥鍼液의 抗酸化 效能에 관한 硏究I 安埈徹;文振榮;林鍾國
  14. 大韓鍼灸學會誌 v.11 濃度別 當歸藥鍼이 放射線 被曝에 의한 免疫機能 低下에 미치는 影響 黃 安秉哲;朴東錫
  15. 생약학회지 v.30 시호 약침제제가 생쥐의 면역활성에 미치는 영향 문진영;임종국;최혜경;이임태;이항우;남경수
  16. 생약학회지 v.30 금은화 약침액의 암예방 효과 김중완;최혜경;손윤희;임종국;이항우;남경수
  17. Anal. Biochem. v.169 Direct measurment of NAD(P)H: Quinone reductase from cells ciltured in microtiter wells: A screening assay for anticarcinogenic enzyme inducers Prochaska. H.J.;Sanamaria. A.B.
  18. Anal. Biochem. v.106 Determination of gluathione and glutathinoe disulfide using glutathione reductase and 2-vinylpyridine Griffith. O.W.
  19. J. Biol. Chem. v.249 Glutathione S-transferase : the first enzymatic step mercapturic acid formation Habig. W.H.;Pabst. M.H.;Jacoby. W.B.
  20. J. Protozool. v.34 Inhibition of multiplication in Acanthamoeba castellanii by specific inhibition of ornithine decarboxylase Kim. G.G.;McCann. P.P.;Byers. T.J.
  21. Biologically Reactive Intermediates Biochemical aspects of toxic metabolites formationl, detoxification and covalent binding Jollow. D.J.;Smith. C.;Jollow. K.J.(ed.);Kocsis. J.J.(ed.);Snyder. R.(ed.);Vainio. H.(ed.)
  22. Gluthathione : Metabolism and Function Glutathione and drug induced tissue lesions Mitchell. J.R.;Hinson. J.A.;Nelson. S.D.;Arias. I.M.(ed.);Jakoby. W.B.(ed.)
  23. Functions of Gluathione: Biochemical, Physiological, Toxicological, Clinical Aspects Interaction of glutathione with reactive intermediates Molders. P.;Jernestiom. B.;Larson. A.(ed.);Orrenius. S.(ed.);Holgren. A.(ed.);Mannervik. B.(ed.)
  24. Anticancer Res v.6 Interference with polyamine biosynthesis and / or function by analogs of polymines or methionine as a potential anticancer chemotherapeutic strategy Porter. C.W.;Sufrin. J.R.
  25. Microbiol. Rev. v.49 Polyamines in microorganisms Tabor. C.W.;Tabor. H.
  26. Am. J. Physiol. v.243 Polyamine metabolism and function Pegg. A.E.;McCann. P.P.
  27. Cancer Res. v.44 Ornithine Decarboxylase activity in the rat and human colon Rozhin. J.;Wilson. P.S.;Bull. A.W.;Nigro. N.D.
  28. Biochem. Biophys. Res. Com- man. v.105 Polyamine biosynthesis and skin tumar promotion: inhibition of 12-o-tetradecanoyl-phorbol-13-acetate-promoted mouse skin tumor formation by the irreversible inhibitor of ornithine decarboxylase-difluoromethylornithine Takigawa. M.;Verma. A.K.;Simsiman. R.C.;Boutwell. R.K.