Archives of Pharmacal Research
- Volume 23 Issue 4
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- Pages.385-390
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- 2000
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- 0253-6269(pISSN)
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- 1976-3786(eISSN)
Effects of Solvent Selection and Fabrication Method on the Characteristics of Biodegradable Poly(lactide-co-glycolide) Microspheres Containing Ovalbumin
- Cho, Seong-Wan (Department of Pharmaceutics, College of Pharmacy, Chung-Ang University) ;
- Song, Seh-Hyon (Department of Pharmaceutics, College of Pharmacy, Chung-Ang University) ;
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Shoi, Young-Wook
(Department of Pharmaceutics, College of Pharmacy, Chung-Ang University)
- Published : 2000.08.01
Abstract
To demonstrate the effect of formulation conditions on the controlled release of protein from poly(lactide-co-glycolide) (PLGA) microspheres for use as a parenteral drug carrier, ovalbumin (OVA) microspheres were prepared using the W/O/W multiple emulsion solvent evaporation and extraction method. Methylene chloride or ethyl acetate was applied as an organic phase and poly(vinyl alcohol) as a secondary emulsion stabilizer. Low loading efficiencies of less than 20% were observed and the in vitro release of OVA showed a burst effect in all batches of different microspheres, followed by a gradual release over the next 6 weeks. Formulation processes affected the size and morphology, drug content, and the controlled release of OVA from PLGA microspheres.