Animal cells and systems

  • 제4권4호
  • /
  • Pages.341-345
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  • 2000
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  • 1976-8354(pISSN)
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  • 2151-2485(eISSN)
한국통합생물학회 (The Korean Society for Integrative Biology)
권호 표지

Characterization of B- , T- , and NK-like Cells in Nile Tilapia (Oreochromis nilotica)

  • Choi, Sang-Hoon (Department of Marine Biomedical Science, Kunsan National University) ;
  • Oh, Chan-Ho (Division of Biotechnology, Woosuk University)
  • 발행 : 2000.12.01
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초록

It has been very difficult to develop and evaluate efficient fish vaccines because fish immune cells have not been properly characterized. In this study, we investigated the cell-mediated immunological properties of B- and T-like cells in Nile tilapia (Oreochromis nilotica). Surface immunoglobulin negative ($slg^{-}$) cell population proliferated in response to mammalian T-cell mitogens PHA and Con A, while surface immunoglobulin positive ($slg^{+}$) cells responded to the B-cell mitogen LPS. The slg$^{[-10]}$ cells from hemocyanin (HC)-immunized Tilapia, compared to the non-immunized control, reacted more to PHA than to Con A. Unexpectedly, antigen (Ag)-specific response was observed in both $slg^{-}$ and $slg^{-}$cells. Regardless of HC immunization, whole leukocytes from 8 head kidney of fish showed natural killer (NK)cell activity. Especially, NK cell activity was much higher in slg$^{[-10]}$ cells than in slg$^{+}$cells, indicating the possibility that fish NK cells were not at least associated with slg$^{+}$ cell population and not activated by Ag. Further understanding of functional fish immune cells will help to evaluate and develop effective vaccines for fishes and to monitor the course of therapy In infected fishes.hes.

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참고문헌

  1. Avtalion RR, Weiss E, and Moalem T (1976) Regulatory effects of temperature in ectothermic vertebrates. In: Marchalonis JJ(ed), Comparative Immunology, Blackwell Scientific Publications, Oxford, pp 227-238
  2. Caspi RR and Avtalion RR (1984) Evidence for the existence of an IL-2-like lymphocyte growth promoting factor in a bony fish, Cyprinus carpio. Dev Comp Immunol 8: 51-60 https://doi.org/10.1016/0145-305X(84)90009-0
  3. Choi SH (1997) The purification of fish B-like cells and their functional properties. Korean J Immunol 19: 113-120
  4. Clem LW, Miller NW, and Bly JE (1991) Evolution of lymphoid subpopulations, their interactions, and temperature sensitivities. In: Warr GW and Cohen N (eds), Phylogenesis of Immune Functions. CRC Press Inc. Boca Raton, pp 111-123
  5. DeLuca D, Wilson M, and Warr GW (1983) Lymphocyte heterogeneity in the trout, Salmo gairdneri, defined with monoclonal antibodies to IgM. Eur J Immunol 13: 546-551 https://doi.org/10.1002/eji.1830130706
  6. Erdal JI and Reitan LJ (1992) Immune response and protective immunity after vaccination of Atlantic salmon (Salmo salar L.) against furunculosis. Fish & Shellfish Immunol 2: 99-108 https://doi.org/10.1016/S1050-4648(05)80039-7
  7. Etlinger HM, Hodgin HO, and Chiller JM (1976) Evolution of the lymphoid system. 1. Evidence for lymphocyte heterogeneity in rainbow trout revealed by the organ distribution of mitogenic responses. J Immunol 116: 1547-1553
  8. Graham S and Secombes CJ (1990a) Do fish lymphocytes secrete interferon gamma? J Fish Biol 36: 563-573 https://doi.org/10.1111/j.1095-8649.1990.tb03557.x
  9. Graham S and Secombes CJ (1990b) Cellular requirements for lymphokine secretion by rainbow trout (Salmo Gairdneri) leucocytes. Dev Comp Immunol 14: 59-68 https://doi.org/10.1016/0145-305X(90)90008-3
  10. Graves SS, Evans DL, Cobb D, and Dawe DL (1984) Nonspecific cytotoxic cells in fish (Ictalurus punctatus). I. Optimum requirements for target cell lysis. Dev Comp Immunol 8: 293-302 https://doi.org/10.1016/0145-305X(84)90036-3
  11. Groundel JL and Harmsen EGM(1994) Phylogeny of interleukins(1994) growth factors produced by leucocytes of the cyprinid fish, Cyprinus carpio L. Immunology 52: 477-482
  12. Marsden MJ, Hamdani SH, and Secombes CJ (1995) Proliferative responses of rainbow trout, Oncorhynchus mykiss, T and B cells to antigens of Aeromonas salmonicida Fish & Shellfish Immunol 5: 199-210 https://doi.org/10.1016/S1050-4648(05)80014-2
  13. Ruben LN, Warr GW, Decker JM, and Marchalonis JJ (1977) Phylogenetic origins of immune recognition: lymphoid heterogeneity and the hapten/carrier effects in the goldfish, Carassius auratus. Cell Immunol 31: 266-283 https://doi.org/10.1016/0008-8749(77)90028-4
  14. Sizemore RG, Miller NW, Cuchens MA, Lob CJ, and Clem LW (1984) Phylogeny of lymphocyte heterogeneity: The cellular requirements for in vitro mitogenic responses of channel catfish leukocytes. J Immunol 133:2920-2924
  15. Smith SA, Gebhard DH, Housman JM, Levy MG, and Noga EJ (1997) Isolation, purification, and molecular-weight determination of serum immunoglobulin from Oreochromis aureus. J Aquat Anim Heal 5: 23-35 https://doi.org/10.1577/1548-8667(1993)005<0023:IPAMWD>2.3.CO;2
  16. Stolen JS and Makela O (1976) Cell collaboration in marine fish: The effect of carrier preimmunization on the anti-hapten response to NIP and NNP, In: Wright RK and Cooper EL(eds), Phylogeny of Thymus and Bone Marrow-Bursa Cells. Elsevier/North Holland, Amsterdam, pp 93-97
  17. Vallejo AN, Miller NW, and Clem LW (1991) Phylogeny of immune recognition: processing and presentation of structurally-defined proteins in channel catfish immune responses. Dev Immunol 1: 137-148
  18. Vallejo AN, Miller NW, and Clem W (1992) Celluar pathway(s) of antigen processing in fish antigen presenting cells: Effect of varying in vitro temperatures on antigen catabolism. Dev Comp Immunol 16: 367-381 https://doi.org/10.1016/0145-305X(92)90039-F