Animal cells and systems

The Korean Society for Integrative Biology (한국통합생물학회)
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Immunohistochemistry of the Pancreatic Endocrine Cells of the Red-eared Slider (Trachemys scripta elegans)

  • Ku, Sae-Kwang (Pharmacology & Toxicology Laboratory, Central Research Laboratories, Dengwha Pharmaceutical Industry Co., Ltd) ;
  • Lee, Hyeung-Sik (Department of Biology, Faculty of Natural Sciences, Kyungsan University) ;
  • Lee, Jae-Hyun (Department of Histology, College of Veterinary Medicine, Kyungpook National University) ;
  • Park, Ki-Dae (Department of Histology, College of Veterinary Medicine, Kyungpook National University)
  • Published : 2000.06.01
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Abstract

Regional distribution and relative frequency of endocrine cells in the pancreas of the red-eared slider, Trachemys scripta elegans, were investigated by immunohistochemical methods. Chromogranin (Cg) A-, serotonin-, insulin-, glucagon-, somatostatin-, bovine pancreatic polypeptide (BPP)- and human pancreatic polypeptede (HPP)-immunoreactive cells were identified in this study. Most of immunoreactive cells in the exocrine and endocrine pancreas (Langerhans islet) were generally spherical or spindle-shaped (open-typed cell), while occasionally cells round in shape (close-typed cell) were found in the basal portion or interepithelial regions of the pancreatic duct. These immunoreactive cells were located in the exocrine, endocrine pancreas and/or basal or interepithelial portion of the pancreatic duct. Serotonin-immunoreactive cells were found in the basal portion of epithelia of the pancreatic duct at a low frequency and interacinar region of the exocrine at a moderate frequency. Insulin-immunoreactive cells were found in the central portion of the endocrine pancreas, interacinar regions of the exocrine pancreas and basal portion of the epithelia of the pancreatic duct at high, moderate and low frequencies, respectively. Glucagon-immunoreactive cells were detected in the periphery of the endocrine pancreas, interacinar region of the exocrine pancreas and basal portion of the epithelia or interepithelia of the pancreatic duct at high, moderate and moderate frequencies, respectively. Somatostatin-immunoreactive cells were dispersed in the whole area of the endocrine pancreas, interacinar regions of exocrine pancreas and basal portion of the epithelia or interepithelia of the pancreatic duct at a moderate frequency. BPP- and HPP-immunoreactive cells were detected in the iinteracinar region of the exocrine pancreas at moderate and hige frequencies, respectively. However, no Cg A- and motilin-immunoreactive cells were detected in this study.

Keywords

References

  1. Agulleiro B, Garcia-Ayala A, and Abad ME (1985) An immunocytochemical and ultrastructural study of the endorine pancreas of Pseudemys Scripta elegans (Chelonia). Gen Comp Endocrinol 60: 95-103 https://doi.org/10.1016/0016-6480(85)90298-9
  2. Bell FR (1979) The relevance of the new knowledge of gastrointestinal hormones to veterinary science. Vet Sci Commun 2: 305-314 https://doi.org/10.1007/BF02291460
  3. Brazeau P, Vale W, Burburs R, Ling N, Butcher M, Rivier J, and Guillemin R (1973) Hypothalamic polypeptide that inhibits the secretion of immunoreactive Pituitary growth hormone. Science 179: 77-79 https://doi.org/10.1126/science.179.4068.77
  4. Buchan AMJ, Lance V, and Polak JM (1983) Regulatory peptides in the gastrointestinal tract of Alligator mississipiensis. An immunocytochemical study. Cell Tissue Res 231: 439-449 https://doi.org/10.1007/BF00222193
  5. DEste L, Buffa R, Pelagi M, Sciceardi AG, and Renda T (1994) Immunohistochemical localization of chromogranin A and B in the endocrine cells of the allimentary of the green frog, Rana esculenta. Cell Tissue Res 277: 341-349 https://doi.org/10.1007/s004410050161
  6. Cohn DV, Elting JJ, Frick M, and Elde R (1984) Selective localization of the parathyroid secretory protein 1/adrenal medulla chromogranin A protein family in a wide variety of endocrine cells of the rat. Endocrinology 144: 1963-1974
  7. Ding WG, Kimura H, Fujimura M, and Fujimura M (1991) Nuropeptide Y and Peptide YY immunoreactivities in the pancreas of various vertebrates. Peptides 18: 1523-1529 https://doi.org/10.1016/S0196-9781(97)00237-4
  8. Dockray GJ (1977) Molecular evolution of gut hormones. Application of comparative studies on the regulation of digestion. Gasteroenterology 72: 344-358
  9. El-Salhy M, Abu-Sinna G, and Wilander E (1983) The endocrine pancreas of asquamate reptile, the desert lizard (Chalcides ocellatus). A histological and immunocytochemical investigation. Histochemistry 78: 391-397 https://doi.org/10.1007/BF00496625
  10. El-Salhy M and Grimelius L (1981) The Endocrine cells of the gastrointestinal mucosa of a Squamata reptile, the grass lizard (Mabuya quinquetaeniata). A histologacal and immunohistological study. Biomedical Res 2: 639-658
  11. El-Salhy M, Winder E, and Lundqvist M (1985) Compparative studies of serotonin-like immunoreactive cells in the digestive tract of vertebrates. Biolmedical Res 6: 371-375
  12. Falkmer S and Van Noordenb S (1983) Ontogeny and Phylogeny of glucagon cell. Handb Exp Pharmacol 66: 81-119
  13. Garcia-Ayala A, Lozano MT, and Agulleiro B (1987) Endocrinepancreas of Testudo graeca L. (Chelonia) in summer and winter: an immunocytochemical and ultrastructural study. Gen Comp Endocrinol 68: 235-248 https://doi.org/10.1016/0016-6480(87)90035-9
  14. Garcia-Ayala A, Lozano MT, and Agulleiro B (1989) Comparative study on the endocrine cells in the pancreas of Mauremys caspica (Che;onia) in summer and study. Gen Comp Endocrinol 75: 363-375 https://doi.org/10.1016/0016-6480(89)90171-8
  15. Guyton AC (1988) Textbook of Medical Physiology. 8th ed. WB Saunders, Philadelphia, pp 801-815
  16. Hsu WH and Crump MH (1989) The endocrine pancreas. In: McDonald LE and Pineda NH(eds), Veterinary Endocrinology and Reproduction, Lea & Febiger, Philadelphia, pp 186-201
  17. Kitamura N, Yamada J, Calingasan NY, and Yamashita T (1984) Immunocytochemical distribution of endocrine cells in the gastro-intestinal tract of the horse. Equine Vet J 16: 103-107
  18. Lozano MT, Garcia-Hernandez MP, Garcia-Ayala A, Elbal MT, and Agulleiro B (2000) Identification of the pancreatic endocrine cells of Pseudemys scripta elegans by immunogold labeling. Gen Comp Endocrinol 117: 163-172 https://doi.org/10.1006/gcen.2000.7394
  19. Morescalchi AM,Gacciolo M, Faraldi G, and Tagliafierro G (1997) The gastro-enteric-pancreatic neuroendocrine system in two reptilian species, Chalcides Chalcides and Zoonosaurus madasscariensis (Sauridae). Eur J Histochem 41: 29-40
  20. Ono K, Yamada J, Pai VD, Kitamura N, Gregio EA, Yamashita T, and Campos VJM (1991) An ultrastructural study on the endocrine pancreas of Caiman latirostris, with special reference to pancreatic motilin cells. Arch Histol Cytol 54: 349-357
  21. Pearse AGE, Polak JM, and Bloom SR (1977) The newer gut hormones. Cellulur sources, physiology, pathology and clinical aspects. Gastroenterology 72: 746-761
  22. Perez-Tomas R, Ballesta J, Pastor LM, Madrid JF, and Polak JM (1989) Comparative immunohistochemical study if the gastroenteropancreatic endocrine system of three reptiles. Gen Comp Endocrinol 76: 171-191 https://doi.org/10.1016/0016-6480(89)90148-2
  23. Putti R, Della-Rodda A, Varano L, Laforgia V, and Cavagnuolo A (1992) An immunocytochemical study of the endocrine pancreas in three genera of lacertids. Gen Comp Endocrinol 87: 249-259 https://doi.org/10.1016/0016-6480(92)90029-J
  24. Rindi G, Buffa R, Sessa F, Tortora O, and Solcia E (1986) Chromogranin A,B and C immunoreactivities of mammalian endocrine cells. Distribution from costrored hormones/prohormones and relationship with argyrophil component of secretory granules. Histochemistry 85: 19-28 https://doi.org/10.1007/BF00508649
  25. Rhoten WB and Smith PH (1978) Localization of four polypeptide hormones in the saurian pancreas. Am J Anat 151: 595-601 https://doi.org/10.1002/aja.1001510410
  26. Sternberger LA (1979) The unlbeled antibody peroxidase antiperoxidase (PAP) method. In: Sternberger LA (ed), Immunocytochemistry, John Wiley & Sons, New York, pp 104-169
  27. Solcia E, Capella C, Vassallo G, and Buffa R (1975) Endocrine cells of the gastric mucosa. Int Rev Cytol 42: 223-286
  28. Solcia E, Usellini L, Buffa R, Rindi G, Villani L, Aguzzi A, and Silini E (1989) Endocrine cells producing regulatory peptides. In: Polak JM(ed), Regulatory Prptides, Birkhauser, Basel, pp 220-246
  29. Trandaburu T, Syed-Ali S, and Trandaburu I (1999) Granin proteins (chromogranin A and secretogrenin IIC23 AND C26-3) in the endocrine of reptiles. Anat Anz181: 489-494
  30. Walsh JH (1987) Gastrointestinal hormones. In: Johnson LR(ed), Physiology of the Gastrointestinal tract, 2nd Ed. vol 1, Raven Press, New York, pp 181-253
  31. Yamada J, Rodrigues MAM, Kitamura N, Pai VD, Yamashita T, Motizuki T, and Yanaihara N (1991) Motilin-immunoreactive cells in the duodenum, pyloric stomach and pancreas of caimans (Caiman latirostris and Caiman Crocodilus, Alligatorinae): a forther comparison using region-specific motilin antisear. Arch Histol Cytol 54: 359-364