미생물학회지 (Korean Journal of Microbiology)

  • 제36권4호
  • /
  • Pages.267-272
  • /
  • 2000
  • /
  • 0440-2413(pISSN)
  • /
  • 2383-9902(eISSN)
한국미생물학회 (The Microbiological Society of Korea)
권호 표지

Bradyrhizobium sp.(Cassia) CN9135의 nodD1 유전자의 크로닝과 염기서열 분석

Cloning and Sequence Analysis of the nodD1 Gene from Bradyrhizobium sp.(Cassia) CN9135

  • 최순용 (한남대학교 이과대학 미생물학과) ;
  • 고상균 (대전대학교 이과대학 생명과학부)
  • 발행 : 2000.12.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

차풀(Cassia nomame)의 뿌리혹 공생세균인 Bradyrhizobium sp.(Cassia) CN9135의 nodD1 유전자를 중합효소 연쇄반응을 통해 크로닝하여 그 염기서열을 조사하였다. 염기서열로부터 유추된 NodD1 단백질은 Bradyrhizobium elkanii와 가장 높은 95%의 상동성을 나타내었다. 뿐만 아니라 nodD2 사이의 intergenic space 부위의 염기서열도 B. japonicum을 포함하는 다른 bradyrhizobia와는 상동성이 거의 없었으나 B. elkanii와는 88%의 높은 상동성을 나타내었다. 우리의 실험 결과는 Bradyrhizobium sp.(Cassia)가 B. elkanii와 매우 근연한 관계임을 보여 주고 있다.

A 1.7-kb fragment containing the nodD1 genes of Bradyrhizobium sp. (Cassia) CN9135 was amplified by PCR with primers based on B. japonicum USDA110. This fragment was cloned and sequenced. Analysis of the sequence showed open reading frames highly homologous to nodD1 from other bradyrhizobial sources. The sequence showed higher homology to nodD1 gene of B. elkanii than to those from b. japonicum. Our results suggest that Bradyrhizobium sp. (Cassia) CN9135 may be more closely related to B. elkanii than to B. japonicum.

키워드

참고문헌

  1. 분자생물학뉴스 v.8 Rhizobia 와 식물의 상호작용: nod 유전자와 Nod인자 고상균
  2. 한국미생물학회지 v.35 시험관내 뿌리혹 생성 실험의 개선된 방법 고상균
  3. Int. J. Syst. Evol. Microbiol. v.50 Bradyrhizobium spp. (TGx) isolates nodulating the new soybean cultivars in Africa are diverse and distinct from bradyrhizobia that nodulate North American soybeans Abaidoo, R.C.;H.H. Keyser;P.W. Singleton;D. Borthakur
  4. The Leguminosae Allen, O.N.;E.K. Allen
  5. MPMI v.7 DNA sequence of the common nodulation genes of Bradyhizobium elkanii and their phylogenetic relationship to those of other nodulating bacteria Dobert R.C.;B.T. Breil;E.W. Triplett
  6. Appl. Environ. Microbiol. v.66 Bradyrhizobium sp. strains that nodulate the leguminous tree Aacacia albida produce fucosylated and partially sulfated Nod factors. Ferro M.;J. Lorquin;S. Ba;K.Sanon;J.C. Prome;C. Boivin
  7. J. Bacteriol. v.178 Bradyrhizobium (Arachis) sp. strain NC92 contains two nodD genes involved in the repression of nodA and a nolA gene required for the efficient nodulation of host plants Gillette, W.K.;G.H. Elkan
  8. MPMI v.5 Structural and functional analysis of two different nodD genes in Bradyrhizobium japonicum USDA 110 Gotterfert, M.;D. Holzhauser;D. Bani;H. Hennecke
  9. Personal data Koh, S.K.
  10. Natural Science(Taejon University) v.8 Isolation and characterization of Bradyrhizobium sp. from Cassia nomame root nodules Lee, K.L.;S. K. Koh
  11. Mol. Microbiol. v.25 Molecular mechanisms of Nod factor diversity Mergaert, P.;M. van Montagu;M. Holsters
  12. Appl. Environ. Microbiol. v.65 Relationships of bradyrhizobia from the legumes Apios americana and Desmodium glutinosum Parker, M. A.
  13. Proc. Natl. Acad. Sci. v.74 DNA sequencing with chain terminating inhibitors Sanger, F.;S. Nicklen;A.R. Coulson
  14. Handbook for rhizobia Somasegaran, P.;H.J. Hoben
  15. A manual for the practical study of root-nodule bacteria Vincent, J.M.
  16. J. Bacteriol. v.173 Studies of the Bradyrhizobium japonicum nodD1 promoter: a repeated structure for the nod box Wang, S.P.;G. Stacey
  17. Symbiosis of plants and microbes(1st ed.) Werner, D.
  18. Int. J. Syst. Bacteriol. v.45 Bradyrhizobium liaoningense sp. nov., isolated from the root nodules of soybeans Xu, L.M.;C. Ge;Z. Cui;J. Li;H. Fan