International Journal of Industrial Entomology and Biomaterials
- Volume 1 Issue 2
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- Pages.131-135
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- 2000
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- 3022-7542(pISSN)
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- 2586-4785(eISSN)
Characterization of Vitellin from the Fireflies, Luciola unmunsana and L. lateralis
- Kim, Seong-Ryul (College of Natural Resources and Life Science, Dong-A University) ;
- Bae, Jin-Sik (College of Natural Resources and Life Science, Dong-A University) ;
-
Jin, Byung-Rae
(College of Natural Resources and Life Science, Dong-A University)
;
- Kim, Jong-Gill (Department of Sericulture & Entomology, The National Institute of Agricultural Science & Technology) ;
- Kim, Keun-Young (Department of Sericultural and Entomological Biology, Miryang National University) ;
- Lee, Sang-Mong (Department of Sericultural and Entomological Biology, Miryang National University) ;
-
Sohn, Hung-Dae
(College of Natural Resources and Life Science, Dong-A University)
- Published : 2000.12.01
Abstract
The vitellin of the fireflies, Luciola unmunsana and L. lateralis was characterized. The vitellin of L. unmunfon is composed of two subunits, designated Vnl (195 kDa) and Vn2 (185 kDa) in SDS-polyacryamide gel electrophoresis. These two subunits of vitellin of L. unmunsana gradually decreased during embryogenesis. As expected, these protein bands were presented in female adult hemolymph and egg extracts, but not in male. The vitellin of L. lateralis is also composed of two subunits, designated Vnl (195 kDa) and Vn2 (180 kDa) in SDS-PAGEi and these two protein bands gradually decreased during embryogenesis. Western blot analysis using each of polyclonal antiserum against vitellins of L. unmunsana and L. lateralis showed that two antisera strongly crossereacted with vitellin subunits of L. unmunsana and L. lateralis, suggesting that vitellins of L. unmunsana and L. lateralis have similarity with each other.