International Journal of Industrial Entomology and Biomaterials
- Volume 1 Issue 1
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- Pages.53-58
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- 2000
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- 3022-7542(pISSN)
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- 2586-4785(eISSN)
Expression of the Gene Encoding Firefly Luciferase Using Bombyx mori Nucleopolyhedrovirus Vector
- Woo, Soo-Dong (School of Agricultural Biotechnology, Seoul National University) ;
- Cho, Kook-Ho (School of Agricultural Biotechnology, Seoul National University) ;
- Jin, Byung-Rae (College of Natural Resources and Life Science, Dong-A University) ;
- Boo, Kyung-Saeng (School of Agricultural Biotechnology, Seoul National University) ;
- Kang, Seok-Kwon (School of Agricultural Biotechnology, Seoul National University)
- Published : 2000.09.01
Abstract
A cDNA encoding the luciferase of firefly Luciola lateralis was cloned downstream from the polyhedrin gene promoter of Bombyx mori nucleopolyhedrovirus and expressed in B. mori cells (BmN-4). The coding soquence for luciferase was inserted into pBmKSK2 rectors) which was reconstructed from the polyhedrin-based transfer vector pBmKSKl by modifying cloning sites. Recombinant virus, BmK2-LUCDF, containing the luciferase gene was selected and purified in BmN-4 cells. The emission of luminescence by luciferase was only detected in BmK2-LUCDF-infected cell extracts. This result indicates that the cloned new luciferase gene of firefly L. lateralis can be expressed efficiently in baculovirus expression system and used as a useful reporter gene.