Journal of Veterinary Clinics (한국임상수의학회지)

The Korean Society of Veterinary Clinics (한국임상수의학회)
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Diagnosis of Salmonella dubin in Korean Native Calves using PCR and Nucleotide Sequences of rfb5 Gene

송아지에 감염된 Salmonella dublin의 PCR 진단과 rfbS 항원단백 유전자의 염기서열분석

  • 김철민 (전북대학교 생체안전성연구소) ;
  • 이영준 (전북대학교 생체안전성연구소) ;
  • 박명규 (전북대학교 생체안전성연구소) ;
  • 최경성 (전북대학교 생체안전성연구소) ;
  • 김민석 (전북대학교 생체안전성연구소)
  • Published : 2000.12.01
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Abstract

An epizootic of calf diarrhea occurred in a Korean native cattle farm located in Chonbuk province. The calves that had either bloody or watery diarrhea were 1 to 30 days old. Some of these animals died during the acute course of the disease. Five calves with predominant clinical signs were examined in more detail. Hematological and serum chemical findings were suggestive of dehydration and nutritional insufficiency. Fecal material from the calve was cultured on/in brilliant green agar (BGA), xylose-lysine deoxycholate (XLD) medium, MacConkey agar, eosin methylene blue (EMB) agar and triple sugar iorn (TSI) A bacteria was isolated. which was subsequently identificed as belonging to Salmonella spp. To differentiate Salmoenlla serotype, rfbs gene of S. dublin was amli- find (720 bp) by multiplex (PCR). The rfbS gene sequences of S, dublin ficld isolate(SDC-1) was com- pared with that off S. dublin(S-37) S, dublin(Ahn et al, 1996), S enteritidis(Ahn et al 1996)and S. typhi (Generbak accession No M29682). The identities of nucleotide sequences were 100%. 99.6%, 99.6%, 97.5% respectively.

Keywords

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