Journal of Microbiology and Biotechnology

  • 제10권6호
  • /
  • Pages.811-816
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  • 2000
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Isolation of Bacterial Strain Antagonistic to Pyricularia oryzae and Its Mode of Antifungal Action

  • Bae, Dong-Won (Department of Agricultural Biology, Gyeongsang National University) ;
  • Lee, Joon-Taek (Department of Agricultural Biology, Genetic Engineering Institute, Gyeongsang National University) ;
  • Son, Dae-Young (Genetic Engineering Institute, Plant Molecular Biology and Biotechnology Research Center, Gyeongsang National University) ;
  • Lee, Eun Sook (Department of Preparatory Oriental Medicine, Kyungsan University) ;
  • Kim, Hee-Kyu (Department of Agricultural Biology, Genetic Engineering Institute, Gyeongsang National University)
  • 발행 : 2000.12.01
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초록

An antagonistic bacterium PM-1 which strongly inhyibits the growth of Pyricularia oryzae was isolated and identified as paenibacillus macerans. The antifungal substances of the strain PM-1 showed the broad antifungal spectra against P.oryzae races. Relating to the localization test, it was found that the antifungal substances existed not only in the cytoplasm but also in the culture supernatant, and importantly the antifungal activity of the latter was stronger than that of the former. The extracellular antifungal substances were extremely heat-stable up to $121^{\circ}C$ for 15 min. The substances were optimally produced at $20^{\circ}C$ and pH 10.0 in a potato dextrose broth. The culture filtrate of the strain PM-1 caused a partial swelling of the mycelia of P.oryzae, and it prevents the normal growth of the fungus as well. This result suggested that the antifungal substances secreted by the strain PM-1 potentially inhibited the germination of P.oryzae.

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