Journal of Microbiology and Biotechnology

  • 제10권5호
  • /
  • Pages.714-720
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  • 2000
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  • 1017-7825(pISSN)
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  • 1738-8872(eISSN)
한국미생물·생명공학회 (The Korean Society for Microbiology and Biotechnology)
권호 표지

Immunoelectron Microscopic Localization and Analysis of Herpes simplex Virus Type 1 Antigens

  • Chung, Charles C. (Department of Biology, Konkuk University) ;
  • Lee, Hyung-Hoan (Department of Biology, Konkuk University) ;
  • Cho, Myung-Hwan (Department of Biology, Konkuk University)
  • 발행 : 2000.10.01
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초록

Antigens of Herpes simplex virus type 1 (HSV-1) strain F were immunoblotted to identify the most immunodominant one, and the localization of this antigen was then studied using immunoelectron microscopy. The 67.8 kDa antigen appeared to be the most immunodominant one in a mouse model, and it showed randomly scattered and partially clustered distribution on the surface of the virion. The localization study was performed using immunogold with polyclonal anti-HSV-1 sera produced from BALB/c mice, and immunofluorescence demonstrated that the viral products in the HSV-2 infected Vero cells were distributed throughout the infected host cell, however, mainly on the surface of the host membrane.

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