Journal of Sericultural and Entomological Science (한국잠사곤충학회지)

Korean Society of Sericultural Science (한국잠사학회)
권호 표지

Trend and Technology of Gene and Genome Research

유전자 및 유전체 연구 기술과 동향

  • 이진성 (코아바이오 생명과학연구소) ;
  • 김기환 (성균관대학교 유전공학과) ;
  • 서동상 (성균관대학교 유전공학과) ;
  • 강석우 (농촌진흥청 농업과학기술원) ;
  • 황재삼 (농촌진흥청 농업과학기술원)
  • Published : 2000.12.01
Download PDF
⟨ Previous Next ⟩

Abstract

A major step towards understanding of the genetic basis of an organism is the complete sequence determination of all genes in target genome. The nucleotide sequence encoded in the genome contains the information that specifies the amino acid sequence of every protein and functional RNA molecule. In principle, it will be possible to identify every protein resposible for the structure and function of the body of the target organism. The pattern of expression in different cell types will specify where and when each protein is used. The amino acid sequence of the proteins encoded by each gene will be derived from the conceptional translation of the nucleotide sequence. Comparison of these sequences with those of known proteins, whose sequences are sorted in database, will suggest an approximate function for many proteins. This mini review describes the development of new sequencing methods and the optimization of sequencing strategies for whole genome, various cDNA and genomic analysis.

Keywords

References

  1. Biotechniques v.16 A smiple substraction method for the isolation of cell-dpecific genes using magnetic monodisperse polymer particles Aasheim, H.C.;Deggerdal, A.;Smeland, E.B.;Homes,E.
  2. Science v.252 Complementary DNA sequecing: expressed sequencing tags and human genome project Adams, M.D.;Kelley, J.M.;Gocayne, J.D.;Dubnick, M.;Polymeropoulos, M.H.;Xiao, H.;Merril, C.R.;Wu, A.;Olde, B.;Moreno, R.F.
  3. Nature v.355 3400 new expressed sequence tags identify divesity of transcripts in human brain Adams, M.D.;Kerlavage, A.R.;Fields, C.;Venter, J.C.
  4. Gene v.81 A novel cDNA/PCR strategy for efficient cloning of small amounts of undefined RNA Akowitz, A.;Manuelidis, L.
  5. Nature v.290 Sequence and organization of the human mitochondrial genome Anderson, S.;Bankierm, A.T.;Barrell, B.G.;de Bruijn,M.H.;Coulson, A.R.;Drouin, J.;Eperon, I.C.;Nierlich, D.P.;Roe, B.A.;Sanger, F.;Schreier, P.H.;Smith, A.J.;Staden, R.;Young, I.G.
  6. Nature v.396 The genome sequence of Rikettsia prowazeki and the origin of mitochondria Anderson,S.G.;Zomorodipour, A.;Andersson, J.O.;Sicheritz-Ponten, T.;Alsmark, U.C.;Podowski, R.M.;Naslund, A.K.;Eriksson, A.S.;Winkler, H.H.;Kurland, C.G.
  7. J.Biochem.Biophys.Methods v.13 A non-radioactive automated method for DNA sequence deter-mination Ansorge, W.;Sproat, B.S.;Stegemann, J.;Schwaer, C.
  8. Proc.Natl.Acad.Sci. v.69 Purification of biologically active globin messenger RNA by chromatography on oligothymidly acid-cellulose Aviv, H.;Leder, P.
  9. Nat.Genet v.4 dbEST database for 'expressed sequence tags' Boguski, M.S.;Lowe, T.M.;Tolstoshev, C.M.
  10. Genome Res. v.6 The end of the beginning the race to begin human genome sequencing Boguski, M.;Chakravarti, A.;Gibbs, R.;Green, E.;Myers, R.M.
  11. Science v.277 Yeast as a model organism Botstein, D.;Chervitz, S.A.;Cherry, J.M.
  12. Proc.Natl.Acad. v.85 Continuous on-line DNA sequencing using sligode-oxynucleotide primers with multiple fluorophors Brumbaugh, J.A.;Middendorg, L.R.;Grone, D.L.;Ruth, J.L.
  13. Nucleic Acids Res. v.25 Direct identification of differetially expressed genes by cycle sequencing and cycle labelling using the differential display PCR primers Buess, M.;Moroni, C.;Hersch, H.H.
  14. Methanococcus jannaschii.Science v.273 Complete genome sequence of the methanogenic archaeon Bult, C.J.;White, O.;Olsen, G.J.;Zhou, L.;Fleischmann, R.D.;Sutton,G.G.;Blake, J.A.;FitzGerald, L.M.;Clayton, R.A.;Gocayne, J.D.;Kerlavage, A.R.;Dougherty,B.A.Tomb, J.F.;Adams, M.D.;Reich, C.I.;Overbeek, R.;Kirkness, E.F.;Weinstock, K.G.;Merrick, J.M.;Glodek, A.;Scott, J.L.;Geoghagen, N.S.M.;Venter, J.C.
  15. Science v.236 Cloning of large segments of exogenous DNA into yeast by means of artifical chromosome vectors Burke, D.T.;Carle, G.F.;Olson, M.V.
  16. Biotechniques v.7 Point mutation analysis in a mammalian gene: rapid preparation of total RNA,PCR amplification of cDNA, and Taq sequencing by a novel method Carothers, A.M.;Urlaub, G.;Mucha, H.;Grunberger,D.;Chasin, L.A.
  17. DNA v.4 Supercoil seqyencing : a fast and sinple method for sequencing plasmid DNA Chen, E.Y.;Seeburg, P.H.
  18. Genomics v.17 Ordered shotgun sequencing, a strategy for integrated mapping and sequencing of YAC clones Chen,E.Y.;Schlessinger, D.;Kere, J.
  19. J.Bacteriol v.127 Deoxyribonucleic acid polymerase from extreme thermophile Thermomus aquaticus Chien, A.;Edgar, D.B.;Trela, J.M.
  20. Biochemistry v.18 Deoxyribonucleic acid from sources enriched in ribonuclease Chirgwin,J.M.;Przybyla, A.E.;MacDonald, R.J.;Rutter, W.J.
  21. Anal.Biochem. v.162 Single-step method of RNA isolation by acid gunaidinium thiocyanate-phenol-cholroform extraction Chomecznski, P.;Sacchi, N.
  22. Nature v.377 A YAC contig map of the human genome Chumakov, I.M.;Rigault, P.;Le Gall, I.;Bellanne-Chantelot, C.;Billault, A.;Guillou, S.;Soularue, P.;Guasconi, G.;Poullier, E.;Gros, I.(et al.)
  23. Nat.Genet. v.9 Positional cloning moves from perditional to traditional Collins, F.S.
  24. Proc.Natl.Acad. v.75 Cosmids:a type of plasmid gene-clon-ing vector that is packageable in vitro in bacteropghage lambda heads Collins, J.;Hojn, B.
  25. Nature v.335 Genome linking with yeast artifical chromosomes Coulson,A.;Waterston, R.;Kiff, J.;Sulston, J.;Kohara, Y.
  26. Bioassays v.13 YACs and C.elegans genome Coulson, A.;Kozono, Y.;Lutterbach, B.;Showkeen, R.;Sulston, J.;Waterston, R.
  27. Science v.278 Exploring the metabolic and genetic control of gene expression on a genomic scale DeRisi, J.L.;Iyer, V.R.;Brown, P.O.
  28. Genomics v.37 Gene-representing cDNA clusters defined by hybridization of 57419 clones from infant brain libraries with short oligonucleotide probe Drmanac, S.;Stavropoulos, N.A.;Labat, I.;Vonau, J.;Hauser, B.;Soares, M.B.;Drmanac, R.
  29. J.Virol. v.28 Detection of mouse mamary tumor virus RNA in BALB/c tumor cell lines of nonviral etilogies Dudley, J.P.;Butel, J.S.;Socher, S.H.;Rosen, J.M.
  30. Cell v.7 Enzymatic in vitro synthesis of globin genes Efstratiadis, A.;Kafatos, F.C.;Maxam,A.M.;Maniatis, T.
  31. Science v.269 Whole genome random sequencing and assembly of heamophilus influenzae Rd. Fleischmann, R.D.;Adams, M.D.;White, O.;Clayton,R.A.;Kirkness, E.F.;Kerlavage, A.R.;Bult, C.J.;Tomb, J.F.;Dougherty, B.A.;Merrick, J.M.
  32. Science v.270 The minimal gene complement of Mycoplsma genitalium Fraser, C.M.;Gocayne, J.D.;White, O.;Adams, M.D.;Clayton, R.A.;Fleischmann, R.D.;Bult, C.J.;Keravage, A.R.;Sutton, G.;Kelley, J.M.
  33. Proc.Natl.Acad.Sci. v.85 Rapid production of full-length cDNAs from rare transcripts: amplification using a single gene-specific oligonucleotide primer Forhman, M.A.;Dush, M.K.;Martin, G.R.
  34. Genome Res. v.8 Alternate polyadenylation in human mRNAs: a large-scale amalysis by EST clustering Gautheret, D.;Poirot, O.;Lopez, F.;Audic, S.;Claverie, J.M.
  35. J.Biol.Chem. v.257 Nucleotide sequences from the adenovirus-2 genome Gingeras, T.R.;Sciaky, D.;Gelinas, R.E.;Bing-Dong, J.;Yen, C.E.;Kelly, M.M.;Bullock, P.A.;Parsons, B.L.;O'Neill, K.E.;Roberts, R.J.
  36. Nature v.387 The yeast genome directory Goffeau, A.(et al.)
  37. Genome Res. v.7 Against a whole-genome shotgum Green, P.
  38. Foresic Sci.Int. v.52 DNA typing of foresic material with mixed genotype using allele-specific enzymatic amplification (polymease chain reaction) Gyllenstein, U.B.;Josefsson, A.;Schemschat, K.;Saldeen, T.;Petterson, U.
  39. Nucleic Acids Res. v.19 Substractive cDNA cloning oligo(dT)30-latex and PCR: isoation of cDNA clones specific to undifferentiated human embryoal carcinoma cells Hara, E.;Kato, T.;Nakada, S.;Sekiya, S.;Oda, K.
  40. Kluwer, Dordrecht.The Nethrlands Palnt genome: Methods for genetic and physical mapping Hauge, B.M.;Goodman, H.M.;Bckmann, J.S.(Eds);Osborn, T.C.(Eds)
  41. Gene v.28 Unidirectional digestion with exonuclease III creates targeted breakpoints for DNA sequencing Henikoff, S.
  42. Nucleic Acids Res. v.17 Porduction of single-stranded DNA templates by exomuclease digestion following the polymerase chain reaction Higuchi, R.G.;Ochman, H.
  43. Nucleic Acids Res. v.24 Sequence analysis of 56 kb from the genome of the bacterium Mycoplasma pneumoniae comparising the dnaA region, the atp operon and a cluster of ribosomal protein genes Higert, H.;Himmelreich, R.;Plagens, H.;Herrmann, R.
  44. Plant.J. v.4 An inventory of 1152 expressed scquence tags obtained by partial sequencing of cDNAs from Arabidopsis thaliana Hofte, H.;Desprez, T.;Amselem,J.;Chiapello, H.;Rouze, P.;Caboche, M.;Moisan, A.;Jourjon, M.F.;Charpenteau, J.L.;Berthomieu, P.(et al)
  45. Nucleic Acids Res. v.19 Isoation of a large number of novel mammalian genes by a differential cDNA library screening strategy Hoog,C.
  46. Genet.Anal.Tech.Appl. v.7 Magentic DNA hybridization properties of oligonucleotide probes attached to superparamagnetic beads and their use in the isolation of poly(A) mRNA from eukaryotic cells Hornes, E.;Korsnes,L.
  47. Nature Acids Res. v.22 Identifying differenves in mRNA expression by representatonal difference analysis of cDNA Hubank, M.;Schatz, D.G.
  48. Nucleic Acids Res. v.17 Direct solid phase sequencing of genomic and plasmid DNA using magnetic beads as solid support Hultman, T.;Stahl, S.;Homess, E.;Uhlen, M.
  49. Biotechniquew v.10 Bidirectional soild-phase sequencing of in vitro-amplified plasmid DNA Hultman,T.;Bergh, S.;Moks, T.;Uhlen, M.
  50. Proc.Natl.Acad.Sci. v.85 DNA sequencing with Thermus aaquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA Innis, M.A.;Myambo, K.B.;Gelfand, D.H.;Brow, M.A.
  51. Nat.Genet. v.6 A new bacterophage P1-ferived vector for the propagation of large human DNA fragments Ioannou, P.A.;Amemiya, C.T.;Games, J.;Kroisel, P.M.;Shizuya, H.;Chen, C.;Batzer, M.A.;De Jong,P.J.
  52. Nucleic Acids Res. v.18 Purification of mRNA directly from crude palnt tissues in 15 minutes using magnetic oligo dT microspheres Jakobsen, K.S.;Breivold, E.;Hornes, E.
  53. Genome sequencing.Curr.Biol. v.6 The complete code for a eukaryotic cell Johnston, M.
  54. Anal.Biochem. v.208 Error analysis in manual and automated DNA sequencing Khurshid, F.;Beck, S.
  55. Proc.Natl.Acad. v.65 Selective elimination of the exonuclease activity of the deoxynucleic acid polymerase from E.coli B by limited protelysis Klenow, H.;Henningsen, I.
  56. Nucleic Acids Res. v.18 An 'equalized cDNA library'by reassociation of short double-stranded cDNAs Ko, M.S.
  57. Genome Res. v.8 Reading bits of genetic information : methods for single-nucleotides polymor-phism analysis Landsgren, U.;Nilsson, M.;Kwok, P.Y.
  58. Nucleic Acids Res. v.25 New energy transfer dyrs for DNA sequencing Lee,L.G.;Spurgeon, S.L.;Heiner, C.R.;Benson, S.C.;Rosenblum, B.B.;Menchen,S.M.;Graham, R.J.;Constantinescu, A.;Upadhya, K.G.;Cassel, J.M.
  59. Genmics v.33 Consortium: an integrated molecular analysis of genome and their expression Leenon, G.;Auffray, C.;Polymeropoulos, M.;Soares, M.B.
  60. Science v.257 Differential display of eukaryotic messenger ENA by means of the plymerase chain reaction Liang,P.;Prdee, A.B.
  61. Proc.Natl.Acad.Sci. v.91 A catalog of genes in the cardiobascular system as identified by expressed sequence tags Liew, C.C.;Hwang, D.M.;Fung, Y.W.;Laurenssen, C.;Cukerman, E.;Tsui,S.;Lee, C.Y.
  62. Nature v.227 Adenine-rich polymer associated with rabbit reticulocyte RNA Lim.L.;Canellakis, E.S.
  63. Science v.243 Polymerase chain reaction with single specificity : analysis of T cell receptor delta chain Loh, E.Y.;Elliott, J.F.;Cwirla, S.;Lanier, L.L.;Davis, M.M.
  64. Trend in Genet v.14 Expressed sequene tags-EST ablishing bridges between genomes Marra, M.A.;Hiller, L.;Waterston, R.H.
  65. Gene v.138 Oligo-capping: a simple method to replace the cap structure of eukaryotic mRNAs with oligonucleotides Maruyama, K.;Sugano, S.
  66. Proc.Natl.Acad. v.74 A new method for sequencing DNA Maxam, A.M.;Gilbert, W.
  67. Nat.Genet v.1 Caenorhabditis elegans expressed sequence tags identify gene families and potential diesase gene homologuous McCombie, W.R.;Adams, M.D.;Kelley, J.M.;FetzGerald, M.G.;Utterback, T.R.;Khan, M.;Dubnick, M.;Kerlavage, A.R.;Venter, J.C.;Fields, C.
  68. Integration and Excision of DNA molecules v.26 Single-stranded frlamentous DNA phage as a carrier for in vitro recombined DNA Messing, J.;Gronenborn,B.;Muller-;Hill,B.;Hofschneider, P.H.;Hofschneider, P.H.(Eds.);Starlinger, P.(Eds.)
  69. Gene v.34 A new strategy to create orfered deletions for rapid nucleotide sequencing Misra, T.K.
  70. Automatd DNA sequencing : Construction of cDNA libraries Moreno-Palanques, R.F.;Fuldner, R.A.
  71. Nucleic Acids Res. v.17 Improved double-stranded DNA sequencing using th linear polymerase chain reaction Murray, V.
  72. Nucleic Acids Res. v.24 Effeicient random eubcloning of DNA sheared in a recirculating point-sink flow system Oefner, P.J.;Hunicke-Smith, S.P.;Chiang, L.;Dietrich, F.;Mulligan, J.;Davis, R.W.
  73. DNA Res v.1 An expressed profile of active genes in human colonic mucosa Okubo, K.;Yoshii, J.;Yokouchi, H.;Kameyama, M.;Matsubara,K.
  74. Nat.Genet. v.2 Large scale cDNA sequencing for analysis of quantitative and qualitative aspecs of gene expression Okubo, K.;Hori, N.;Matoba, R.;Niiyama, T.;Fudushima, A.;Kojima, Y.;Matsbara, K.
  75. Trend in Genet v.12 A betwork approach to the systemaic analysis of yeast gene function Oliver, S.
  76. Proc.Natl.Acads. v.83 Random-clone strategy for genomic restriction mapping in yeast Olson, M.V.;Dutchik, J.E.;Graham,M.Y.;Brodeur, G.M.;Helms, C.;Frank, M.;MacCollin, M.;Scheinman, R.;Frank, T.
  77. Proc.Natl.Acad. v.88 Construction of a uniform-abundance cDNA library Patanjali, S.R.;Parimoo, S.;Weissman, S.M.
  78. Science v.238 A system for rapid DNA sequencing with fouroscent -terminating dideoxynucleotides Prober, J.M.;Trainor, G.L.;Dam, R.J.;Hobbs, F.W.;Robertson, C.W.;Zagursky, R.J.;Cocuzza, A.J.;Jensen, M.A.;Baumeister,K.
  79. Genetics v.134 Physical maps of the six smallest chromosomes of Sac-chromyces cecevisiae at a resolution of 2.6 kilobase pairs Riles, M.A.;Dutchik, J.E.;Baktha, A.;McCauley, B.K.;Thauer, E.C.;Leekie, M.P.;Braden, V.V.;Depke, J.E.;Olson, M.V.
  80. Science v.281 A sequencing method based in real-time pyrophosphate Ronaghi,k M.;Uhlen, M.;Nyren, P.
  81. A new resource to facilitate gene idenification. Palnt Physiol. v.112 The construction of Arabidopsis expressed tag assemblies Rounsley,S.D.;Glodek, A.;Sutton, G.;Adams.M.D.;Somerville, C.R.;Venter, J.C.;Kerlavage, A.R.
  82. Science v.230 Enzymativc amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia Saiki,R.K.;Scharf, S.;Fallona, F.;Mullis, K.B.;Horn, G.T.;Erlich, H.A.;Arnheim, N.
  83. Proc.Natl.Acad. v.74 DNA sequencing with chain-terminating inhibitors Sanger, F.;Nicklen, S.;Coulson, A.R.
  84. J.Mol.Biol. v.162 Nucleotide sequence of bacteriophage lambda DNA Sanger, F.;Coulson, A.R.;Hong, G.F.;Hill, D.F.;Peterson, G.B.
  85. Nucleic Acids Res. v.16 RNA amplification with transcript sequencing(RAWTS) Sarker, G.;Sommer, S.S.
  86. Genomics v.14 Detection and cahracterization of chineric yeast artifical chromosome clones by flurorecent in situ suppression hybridization Selleri, L.;Eubanks, J.H.;Giovannini, M.;Hermanson,G.G.;Romo, A.;Djabali, M.;Maurer, S.;McElligott, D.L.;Smith, M.W.;Evans, G.A.
  87. Cell v.9 Capping of eukaryotic mRNA Shatkin, A.J.
  88. Proc.Natl.Acad. v.93 Linking genome and proteome by mass wpectrometry : large-scale identification of yeast proteins from two dimensional gels Shevchenko A.;Jensen,O.N.;Podtelejnikov, A.V.;Sagliocco, F.;Wilm, M.;Vorm, O.;Mortensen, P.;Borcherie, H.;Mann, M.
  89. Nucleic Acids Res. v.16 Lambda ZAP:a bacteriophage lambda expression vector with in vivo exeision propreties Short, J.M.;Femandez, J.M.;Sorge, J.A.;Huse, W.D.
  90. DNA seq. v.1 Seminautomated preparing of DNA templates for large-scale sequencing projects Smith, V.;Brown, C.M.;Bankier, A.T.;Barrell, B.G.
  91. Proc.Natl.Acad. v.91 Construction and characterization of a nomalized cDNA library Soraes, M.B.;Bonald, M.F.;Jelene, P.;Su, L.;Lawton, L.;Efstratiadis, A.
  92. Nucleic Acids Res. v.16 Solid phase DNA sequencing using the biotin-avidin system Stahl, S.;Hultman, T.;Olsson, A.;Morks, T.;Uhlen,M.
  93. Proc.Natl.Acad v.87 Bacteriphage P1 cloning system for the isolation,amplficatiom,and recovery of DNA fragments as large as 100 kilobase pairs Sternberg, N.
  94. Anal. Biochem. v.154 Specific-primer-directed DNA sequencing Strauss, F.W.;Kobori, J.A.;Siu, G.;Sommer, S.S.
  95. Proc.Natl.Acad v.86 A strategy for high-volumn sequencing of cosmid DNAs: ramdom and directed priming with a library of oligonucleotides Studier, F.W.
  96. Nature v.356 The C.elegans genome sequencing project:a begining Sulston, J.;Du.Z.;Thomas, K.;Wilson,R.;Hillier, L.;Staden, R.;Halloran, N.;Green, P.;Thierry-Mieg, J.;Qiu, L.
  97. Gene v.90 Proposal for sequencing DNA using ligation of hexamers to generate sequencial elongation primers(SPL-6) Szybalski, W.
  98. Proc.Natl.Acad.Sci. v.92 A single residue in DNA polymerases of the E.coli DNA polymerase I family is crifical for distinguishing between deowy- and dideoxyribonucleotide Tabor, S.;Richardson, C.C.
  99. Yeast v.11 Construction of a complete genomic library of Saccharomyces cerevisiae and physical mapping of chromosome XI at 3.7kb resolution Thierry, A.;Gaillon, L.;Galibert, F.;Dujon, B.
  100. Anal.Chem. v.64 Solid-pgase nethod for the purification of DNA sequencing reaction Tong, X.;Smith, L.M.
  101. Science v.270 Serial analysis of gene expression Velculescu, V.E.;Zhang,L.;Vogelstein, B.;Kinzler, K.W.
  102. Science v.280 Shotgun Sequencing of the human genome Venter, J.C.;Adams, M.D.;Sutton, G.G.;Keravage, A.R.;Smith, H.O.;Hunkapiller, M.
  103. Gene v.19 The pUC plasmids, an M13mp7-derived system for insertion mutagenesis and sequencing with wynthetic universal primers Vieira, J.;Messing J.
  104. J.Biotechnol v.41 Efficient low redundancy large-scale DNA sequencing at EMBL Voss, H.;Schwager, J.
  105. Nat.Genet v.1 A survey of expressed genes in Caenorhabditis elegans Waterson, R.;Martin, C.;Craxton, M.;Huynh, C.;Coulson, A.;Hillier, L.;Durbin, R.;Green, P.;Shownkeen, R.;Halloran, N.
  106. Genome Res. v.7 Human shole-genome shotgum sequencing Weber, J.L.;;Myers, E.W.
  107. Nucleic Acids Res. v.20 Arbitrarily primed PCR fingerfinting of RNA Welsh, J.(et al.)
  108. Yeast v.9 Sequencing and analysis of 51.6 kilobases on th left arm of chromosome XI from Saccharomyces cerevisiae reveals 23 open reading frames including the FASI gene Wiemann, S.;Voss, H.;Schwager, C.;Rupp, T.;Stegeman, J.;Zimmermann J.;Grothues, D.;Sensen, C.;Erfle, H.;Hewitt, N.
  109. Nucleic Acids Res. v.19 Use of 3' untranslated sequences of human cDNAs for rapid chromosome assignment and conversion to STSs: implification for an expression map of the genome Wilcox, A.S.;Khan, A.S.;Hopkins, J.A.;Sikela, J.M.
  110. Genomics v.13 Nucleotide sequence analysis of 95 kb near the 3' end of the murine T-cell receptor alpha/delta chain locus: strategy and methodology Wilson, R.K.;Koop, B.F.;Chen, C.;Halloran, J.A.;Sikela, J.M.
  111. Nature v.368 2.2Mb of contiguous nucleotide sequence from chromosome III of C. elegans Wilson, R.;Ainscough, R.;Anderson, K.;Baynes, C.;Berks, M.;Bonfiled, J.;Connell, M.;Copsey , T.;Cooper, J.
  112. Nucleic Acids Res. v.18 A simplified protocol for fast plasmid DNA sequencing Zimmermann, J.;Voss, H.;Schwager, C.;Stegemann, J.;Erfle, H.;Stucky,K.;Kristenson, T.;Ansorge,W.