The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Cloning and Expression of a Rice cDNA Encoding a Lls1 Homologue of Maize

  • Jwa, Nam-Soo (School of Agricultural Biotechnology, Research Center, New Bio-Materials in Agriculture, Seoul National University) ;
  • Park, Sang-Geun (School of Agricultural Biotechnology, Research Center, New Bio-Materials in Agriculture, Seoul National University) ;
  • Park, Chan-Ho (School of Agricultural Biotechnology, Research Center, New Bio-Materials in Agriculture, Seoul National University) ;
  • Kim, Soon-Ok (School of Agricultural Biotechnology, Research Center, New Bio-Materials in Agriculture, Seoul National University) ;
  • Ahn, Il-Pyung (School of Agricultural Biotechnology, Research Center, New Bio-Materials in Agriculture, Seoul National University) ;
  • Park, Sook-Young (School of Agricultural Biotechnology, Research Center, New Bio-Materials in Agriculture, Seoul National University) ;
  • Yoon, Choong-Hyo (Molecular Genetics Division, National Institute of Agricultural Science and Technology) ;
  • Lee, Yong-Hwan (School of Agricultural Biotechnology, Research Center, New Bio-Materials in Agriculture, Seoul National University)
  • Published : 2000.06.01
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Abstract

A cDNA (Oslls1) encoding Lls1-homologue of maize was isolated from cDNA library of rice (Oryza sativa cv. Ilpum). The 2,138 bp of full length Oslls1 clone contains an open reading frame of 1,623 nucleotides encoding 575 amino acid residues. The deduced amino acid sequence of Oslls1 has a high level of homology with chlorophyll a oxygenases of Arabidopsis thaliana (67%) and Marchantia polymorpha (65%). Southern blot analysis of genomic DNA indicates the existence of a small gene family for Oslls1 in the rice genome. The expression of Oslls1 mRNA was induced in leaves and germinating seeds. Treatment of $H_2O$$_2$significantly down-regulated Oslls1 expression. The expression of Oslls1 mRNA was consititutively down-regulated in the blm, a rice mutant exhibiting spontaneous necrotic lesions. These results suggest that this Oslls1 gene may be involved incell death mechanisms in the blm mutant of rice.

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