Journal of environmental and Sanitary engineering (환경위생공학)

Korean Society for Environmental Sanitary Engineers (대한환경위생공학회)
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Raped Detection Method for Pathogenic Yersinia enterocolitica by a Multiplex-PCR

Multiplex-PCR을 이용한 병원성 Yersinia enterocolitica의 신속검출 기법에 관한 연구

  • Lee, Young-Kee (Microbial Inspection Team, Seoul Metropolitan Government of Institute of Health and Environment) ;
  • Choi, Sung-Min (Microbial Inspection Team, Seoul Metropolitan Government of Institute of Health and Environment) ;
  • Oh, Soo-Kyung (Microbial Inspection Team, Seoul Metropolitan Government of Institute of Health and Environment) ;
  • Shin, Jei-Young (Microbial Inspection Team, Seoul Metropolitan Government of Institute of Health and Environment) ;
  • Ryeom, Kon (Department of Microbiology, Dankook University)
  • 이영기 (서울특별시보건환경연구원 미생물검사팀) ;
  • 최성민 (서울특별시보건환경연구원 미생물검사팀) ;
  • 오수경 (서울특별시보건환경연구원 미생물검사팀) ;
  • 신재영 (서울특별시보건환경연구원 미생물검사팀) ;
  • 염곤 (단국대학교 미생물학과)
  • Published : 2000.12.01
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Abstract

We have isolated 65 strains(2.0%) of Y. enterocolitica among 3,219 water samples from 380 spring water sites in Seoul from 1994 to 1999. The biochemical characteristics of isolated strains revealed that TSI was A/A, urea, M.R.($37^{\circ}C$), nitrate, motility($37^{\circ}C$), sorbitol, maltose, manitol, arabinose, mannose, trehalose, xylose were positive(100%) and H$_2$S, arginine, lysine, oxidase, citrate, V.P.($37^{\circ}C$), DNase, motility($37^{\circ}C$), dulcitol, adonitol, lactose and raffinose were negative(100%). In in vitro virulence test, positive rate of AAG and CRMOX were 9.2% and 4.6%, respectively. However in the virulence gene detectable gene detectable test by multiplex-PCR using ail, yst, virF genes, 65 strains were all negative, meaning that Y.enterocolitica strains from domestic spring water were not detected for the virulence. Otherwise, mutiplex-PCR which using ail, yst and subgenus-specific primer pair was the best for identifying the virulence of Y. enterocolitica.

Keywords

References

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