Biodetoxification of Coumaphos Insecticide Using Immobilized Escherichia coli Expressing Organophosphorus Hydrolase Enzyme on Cell Surface

  • Mansee, Ayman H. (On leave from Pesticide Chemistry Department, Faculty of Agriculture(El-Shaby), Alexandria University) ;
  • Chen, Wilfred (Department of Chemical and Environmental Engineering, University of California) ;
  • Mulchandani, Ashok (Department of Chemical and Environmental Engineering, University of California)
  • 발행 : 2000.11.01

초록

Recently, we reported an improved technology for the degradation of organophosphate nerve agents using whole cells of genetically engineered Escherichia coli that anchored and displayed the enzyme organophosphorus hydrolase on the cell surface. In this paper we report the immobilization of these cells on highly porous sintered glass beads and the subsequent application of the immobilized cell in a continuous-flow packed bed bioreactor for the biodetoxification of a widely used insecticide, coumaphos.

키워드

참고문헌

  1. FAO Prod. Yearb. v.43 Rome Food and Agricultural Organization of the United Nations
  2. Agricultural statistics United States Department of Agriculture
  3. Pesticides industry sales and usages: 1994 and 1995 market estimates Environmental Protection Agency
  4. Pestic. Sci. v.48 Biodegradation of organophosphate insecticide coumaphos in highly contaminated soil and in liquid wastes Mulbury, W. W.;P. L. Del Valle;J. S. Karns
  5. Biotechnol. Bioeng. v.37 Detoxification of organophosphate pesticides using an immobilized phosphotriesterase from Pseudomonas diminuta Caldwell, S. R.;F. M. Raushel
  6. World J. Microbiol. Biotechnol. v.12 Methylparathion degradation by Pseudomonas sp. A3 immobilized in sodium alginate beads Ramanathan, M. p.;D. Lalithakumari
  7. Nature Biotechnol. v.15 Biodegradation of organophosphorus pesticides by surface-expressed organophosphorus gydrolase Richins, R.;I. Kaneva;A. Mulchandani;W. Chen
  8. Trends Biotechnol. v.16 The use of live biocatalysts for pesticide detoxification Chen, W.;A. Mulchandani
  9. Biotechnol. Bioeng. v.63 Detoxification of organophosphate nerve agents by immobilized Escherichia coli with surface-expressed organophosphorus hydrolase Mulchandani, A.;I. Kaneba;W. Chen
  10. J. Biol. Chem. v.261 Purification and properties of the phosphotriesterase from Pseudomonas diminuta Dumas, D. P.;S. R. Caldwell;J. R. Wild;F. M. Raushel
  11. Groundwater v.20 In situ surfactant washin of polychlorinated biphenyls and oil from a contaminated site Abdul, A. S.;T. L. Gibson;C. C. Ang;J. C. Smith;R. E. Sobczynski
  12. Bioremediation J. v.2 An Integrated surfactant solubilization and PCB bioremediation process for soils Layton, A. C.;C. A. Lajoie;J. p. Easter;M. Muccini;G. S. Sayler
  13. Appl. Environ. Microbiol. v.61 Biodegradation of napthalene in aqueous nonionic surfactant systems Liu, Z.;A. M. Jacobson;R. G. Luthy
  14. Appl. Environ. Microbiol. v.64 Construction of a bioluminescent reporter strain to detect polychlorinated biphhenyls Layton, A. C.;M. Muccini;M. M. Gosh;G. S. Sayler