Biotechnology and Bioprocess Engineering:BBE

  • 제5권5호
  • /
  • Pages.332-339
  • /
  • 2000
  • /
  • 1226-8372(pISSN)
  • /
  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

Separation of $\Phi$X HAE III DNA with Electrochromatography

  • 발행 : 2000.09.01
PDF 다운로드
⟨ 이전 논문 다음 논문 ⟩

초록

Experimental and theoretical works were performed for the separation of large polyelectrolytes such as DNA in the column packed with gel particles under an electric field. This paper shows how intraparticle convection effects the separation of DNAs in the column because DNAs quickly oriented through the pores in the field direction. Dimensionless transient mass balance equations were derived considering diffusion and electrophoretic convection. The separation criteria is theoretically studied using two different Peclet numbers in the fluid and solid phases and these criteria were verified uing two different DNAs by electrophoretic mobilities measured experimentally, showing how the separation position of DNAs varies in the column according to values of Pe(sub)f/Pe(sub)g of individual DNA. Governing equations are simultaneously solved by operator theoretic and characteristic methods to yield the column response.

키워드

참고문헌

  1. Chem. Eng. Sci v.32 Simultaneous intraparticle forced convection, diffusion and reaction in a porous catalyst I Nir, A.;L. Pisman
  2. Chem. Eng. Sci v.44 The effect of intraparticle convection on nutrient trasport in porous biological pellets Staphanopoulos, G.;K. Tsiveriotis
  3. Biotechnol. Prog v.9 Effect of intraparticle convection on the chromatography of biomolecules Frey, D. D.;E. Schweinheim;C. Horvath
  4. Chem. Eng. Sci v.50 Linear driving force approximation for intraparticle diffusion and convection in permeable supports Carta, G.
  5. Ind. Eng. Chem. Res v.32 Effect of intraparticle forced convection on gas desorption from fixed beds containing Large-pore ad-sorbents Lu, Z.;J. M. Lourero;M. D. Douglas;A. E. Rodrigues
  6. Biochen. Eng. J. v.1 Mass transfer mechanisms in Hyper D media for chromatographic protein separation de la Vaga, R.;C. Chenou;J. M. Loureiro;A. E. Rodrigues
  7. Biochem. Eng. J v.2 Dynamic behabior of a fixed-bed biofilm reactor;analysis of the role of the intraparticle convective flow under biofilm growth Leitao, A.;A. Rodrigues
  8. AIChE J. v.39 Solute retention in electrochromatography by electrtically in duced sorption Rudge, S. R.;S. K. Basak;M. R. Ladish
  9. Sep. Sci. Tech v.33 A new approach to counteracting chromatographic electrophoresis Yang, T;C. J. Lee;I. M. Chu
  10. Electrochemical System Newman, J. S.
  11. Sep. Sci. Tech v.27 Electric field driven separations;Phenomena and applications Ptassinski, K. J;P. J. A. M. Kerkhof
  12. Linear Operator Methods in Chemical Engineering with Applications to Transport and Chemical Reaction System Ramkrishna, D.;N. Amundson
  13. Chem. Eng. Sci. v.48 Application of selfadjoint operators to electrophoretic transport in a composite media Ⅱ Locke, B.;P. Arce;Y. Park
  14. Theory and Application of Single Equation v.Ⅰ First-order Partial Differential Equations Rhee, H.;R. Aris;N. Amundson
  15. Biololymer v.2 Electrophoresis of nucleic acids Olivera, B.;P. Baine;N. Davison
  16. Chem. Eng. Sci. v.46 Residence time destribution of inert and linearly adsorbed species in a fixed bed containing large-pore supports;Applications in separation engineering Rodrigues, A. E.;L. Zupping;J. M. Loureiro
  17. Science v.251 DNA;a model compound for solution studies of macromolecules Pecora, R
  18. AIChE J v.9 Hindered transport of large molecules in liquid-filled pores Deen, W. M.
  19. Elements of Chemical Reaction Endgineering Fogler, H. S
  20. Biopolymer v.25 On the reptation thory of gel electrophoresis Slater, G. W.;J. Noolandi
  21. Bipolymer v.26 Electrophoretic mobility of λ phage DNA fragments in agarose gel Hervet, H;C. P. Bean