KSBB Journal

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
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Analysis of Avidin-biotinylated Liposome Layers on Au Electrode by Quartz Crystal Analyzer

수정진동자를 이용한 Au 표면에서 avidin-biotin 결합 리포좀 막의 형성구조 분석

  • Song, Seong-Hun (Department of Chemical Engineering, Dong-A University) ;
  • Cho, Hong-Sig (Department of Chemical Engineering, Dong-A University) ;
  • Park, Jong-Won (Department of Chemical Engineering, Dong-A University) ;
  • Kim, Kwang (Department of Chemical Engineering, Dong-A University) ;
  • Nakamura, Chikashi (Tissue Engineering Group, National institute for Advanced Interdisciplinary Research) ;
  • Yang, Qing (Tissue Engineering Group, National institute for Advanced Interdisciplinary Research) ;
  • Miyake, Jun (Tissue Engineering Group, National institute for Advanced Interdisciplinary Research) ;
  • Chang, Sang-Mok (Department of Chemical Engineering, Dong-A University)
  • 송성훈 (동아대학교 화학공학과) ;
  • 조홍식 (동아대학교 화학공학과) ;
  • 박종원 (동아대학교 화학공학과) ;
  • 김광 (동아대학교 화학공학과) ;
  • 중촌사 (일본 공업대학원 산업기술융합영역연구소) ;
  • ;
  • 삼댁순 (일본 공업대학원 산업기술융합영역연구소) ;
  • 장상목 (동아대학교 화학공학과)
  • Published : 2000.10.01
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Abstract

Liposomes and proteoliposomes, artificial membranes, can interact with many solutes, such as drugs, peptides and proteins. The immobilization of (prot대)liposomes as supramolecular aggregates on gold surfaces have potential applications in nano and biosensor technology. We demonstrated a quartz crystal analyzer (QCA) based method to monitor the construction of multi-layers of unilamellar liposomes based on avidin-biotin binding on gold surfaces using a quartz crystal microbalance (QCM). Thus, the QCA provides an on line and efficient method of detecting the construction of protein membranes, which has applications in biosensing systems.

Avidin의 금속표변에 대한 강한 결합력과 avidin-biotin의 강한 결합력을 이용하여 금속 표면 위에 라포좀과 같은 유기 분자막의 다층 형성 과정을 수정진동자를 이용하여 분석하였다. 금속 표변위에 적층되는 유기 분자막에 대한 정보를 수 집하고 그 가능성을 검토하여 바이오 센서에서 감도를 향상 시킬 수 있는 새로운 방법을 제시하고자 하였다.

Keywords

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