Environmental Mutagens and Carcinogens (한국환경성돌연변이발암원학회지)
- Volume 20 Issue 1
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- Pages.7-13
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- 2000
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- 1225-6307(pISSN)
Effect of Antioxidants and Chelating Agents on 1,2,4-benzenetriol-induced DNA damage in HL-60 cells analysed by alkaline comet assay
항산화제 및 금속착화합물이 1,2,4-benzenetriol에 의해 유도된 HL-60 세포의 DNA 손상에 대한 보호 효과
Abstract
The mechanisms of benzene toxicity is not fully elucidated, although the metabolism of benzene is very well understood. In order to study the mechanism of benzene toxicity, we investigated DNA damage induced by benzene metabolite, 1,2,4-benzenetriol (BT) in HL-60 cells by alkaline comet assay. To investigate the mechanism of cellular DNA damage induced by BT, the cells were treated with antioxidant such as vitamin C, SOD, catalase, and chelating agent such as deferoxamine (DFO), bathocuproinedisulfonic acid (BCDS). BT induced DNA damage in dose-dependent manner at concentration between 10
Keywords
- 1,2,40benzenetriol (BT);
- alkaline comet assay;
- Reactive Oxygen Species (ROS);
- vitamin C;
- SOD;
- catalase;
- deferoxamine (DFO);
- bathocuproinedisulfonic acid (BCDS)