characteristics of Biosurfactant Produced by Pseudomonas sp. EL-G527 from Activated Sludge

  • Lim, Eun-Gyoung (Department of Microbiology Pusan National University) ;
  • Cha, Mi-Sun (Department of Microbiology Pusan National University) ;
  • Park, Geun-Tae (Department of Microbiology Pusan National University) ;
  • Son, Hong-Joo (Department of Biotechnology Miryang National University) ;
  • Lee, Sang-Joon (Department of Microbiology Pusan National University)
  • Published : 2000.12.01

Abstract

Pseudomonas sp. EL-G527 was grown to produce a biosurfactant on 2% n-hexadecane as the energy and carbon source. This biosurfactant significantly reduced the surface tension of water from 72 to 28 dyne/cm at a critical micelle concentration(CMC) of 140 mg/l at pH 2.0. As the pH value decreased, the reduction in the surface tension due to the biosurfactant increased. The surface activity of the biosurfactant was unaffected when the NaCl concentration was increased to 5% and the calcium ion concentration increased to 100 mM, plus it remained stable at 10$0^{\circ}C$ for 180 min.

Keywords

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