Growth Responses at Different Growth Stage of Pinus densiflora Seedlings to Enhanced Uv-B Radiation

자외선-B 증가에 따른 소나무 유묘의 생장 단계별 생장 반응

This study was carried out to investigate the growth responses of Pinus densiflora seedlings to enhanced UV-B environment for 16 weeks in the field condition. The seedlings were treated with one of three levels of UV-B dosages - ambient UV-B, ambient + 3.2, and ambient + 5.2 KJ m$^{-2}$day$^{-1}$ and the irradiation was performed at the stage before the germination, the fully expanded cotyledon, and the primary needles grown more than 0.8cm in length of the seedlings, respectively. Enhanced UV-B irradiation reduced the height and the root collar diameter growth, and dry mass production of the seedling, and T/R ratio was increased by the UV-B treatment. Difference in seedling growth was observed by difference in time of the UV-B treatment. Among the seedlings which were treated with ambient - 3.2 KJ m$^{-2}$day$^{-1}$, height and root collar diameter growth was relatively high in the seedling received the UV-B treatment at the stage before the germination. The lowest dry mass production was observed in the seedlings received the UV-B at stage of cotyledon both in two levels of enhanced UV-B treatment. Chlorophyll concentration was reduced by enhanced UV-B irradiation, and chlorophyll a/b ratio was increased by the UV-B treatment.

본 연구는 소나무(Pinus densifora S. et Z.) 유묘의 발아전 단계, 자엽 단게 및 1차엽 발생후 단계 등 각각의 생육단계에서의 UV-B 증가 환경에 노출될 때의 생장반응을 탐구하고자 16주 동안 3수준의 UV-B(ambient UV-BBE, ambient + 3.2, ambient + 5.2 KJm-2s-1) 조사량이 설치된 야외 포장에서 실시되었다. UV-B 조사에 의하여 소나무 유묘의 수고생장, 근원경생장 및 건물생산량은 감쇠되었으며 T/R율은 증가하였다. 유묘에 대한 UV-B 처리시작 단계 차이에 따른 생장차이도 관찰되었는데, ambient +3.2 처리구내에서 보면 발아전 단계부터 UV-B 처리를 받은 유묘의 수고생장 및 근원경생장이 상대적으로 높았다. 주 수준의 UV-B 증가 처리구에서 모두 자엽단계부터 처리를 잗은 유묘의 건물생산량이 각 처리구내에서 가장 낮앗다. 한편 UV-B 증가에 의해 소나무 유묘 침엽의 엽롭소 함량은 감소하였으며 엽록소 a/b율은 증가하였다.