Comparison of Sensitivity for Detection of Heat-Labile Enterotoxin of Enterotoxienic Escherichia coli(EC 81) and Enterotoxin of Enterotoxigenic Clostridium perforngens type A (NCPC8238) by Means of a Polymerase Chain Reaction Assay

독소원성 대장균(EC81)이 생산하는 이열성장독소와 Clostridium perfringens A형 (NCTC8238)이 생산하는 장독소의 검색을 위한 중합효소 연쇄반응기법의 감도 비교

Detection for heat-labile enterotoxin(LT) of enterotoxigenic Escherichia coli(ETEC, EC81, O148:H28) and enterotoxin of enterotoxigentic Clostridium perfringents type A(CP, NCTC8238, Hobbs serotype 2) by use of a polymerase chain reaction (PCR) assay were positive reaction, which using LT gene-specific primers of ETEC with a detection limit equivalent from 100ng/${\mu}\ell$ to 1 pg of a DNA fragment of 417-bp in EC81 and enterotoxin gene-specific primers of CP with a detection limit equivalent from 100ng/${\mu}\ell$ to 10pg of a DNA fragment of 364-bp in NCTC8238. Detection for a LT gene of ETEC highly appeared 10-fold sensitivity than an enterotoxin gene of CP.