Journal of Embryo Transfer (한국수정란이식학회지)

The Korean Society of Embryo Transfer (한국수정란이식학회)
권호 표지

Effect of Hyper tonic Solution on In Vitro Development of Hanwoo Blastocysts Produced In Vitro

체외에서 생산된 하우 배반포의 발달에 미치는 삼투압의 효과

  • Kim, J. Y. (Department of Biotechnology, Taegu University) ;
  • Cho, Y. H. (Department of Biotechnology, Taegu University) ;
  • Kong, K. H. (Department of Animal Science, Taegu University) ;
  • Yoon, S. H. (Maria Obstertics and Gyecology) ;
  • Lee, S. M. (Hana Women's Hospital) ;
  • Lee, S. J. (Department of Animal Science, Sahmyook College) ;
  • Song, H. B. (Department of Animal Science, Taegu University) ;
  • Park, H. D. (Department of Biotechnology, Taegu University)
  • 김재영 (대구대학교 생물공학과) ;
  • 조영호 (대구대학교 생물공학과) ;
  • 공건오 (대구대학교 축산학과) ;
  • 윤산현 (마리아 기초의학연구소) ;
  • 이상민 (하나병원) ;
  • 이상진 (삼육대학교 동물자원학과) ;
  • 송해범 (대구대학교 축산학과) ;
  • 박흠대 (대구대학교 생물공학과)
  • Published : 2000.02.01
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Abstract

This study was conducted to enhance the efficiency of embryo transfer and embryos produced in vitro according to select good quality blastocyst among in vivo or in vitro produced embryos. After middle blastocys(MB) and late blastocyst(LB) stages embryos that developed in vitro for 7∼8 days were treated hypertonic solution(380 mOsm or 600 mOsm) and the rate of development to hatched blastocyst(HB) was examined. The results obtained were summarized as follows: The proportion of embryos developed to HB was higher than that of control regardless of hypertonic solution treatment. However, it was related to culture time in hypertonic solution The propotrion of embryos developed to HB of morphologically recovered embryos in 380 nOsm solution ws higher than that of morphologically shrinked embryos. However, treatment time was not significantly different the rates of HB development. Especially, the proportion of embryos developed to HB of morphologically recovered embryos of the 30-min-treated group was significantly higher than that of control (p<0.05). The results suggest that hypertonic solution treatment should enhance the efficiency for criterion of transferable blastocyst quality.

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