Biotechnology and Bioprocess Engineering:BBE

The Korean Society for Biotechnology and Bioengineering (한국생물공학회)
권호 표지

High-Performance liquid Chrmatogrphic and Tandem Mass Spectrometric Quantitation of N7-Methyldeoxyguanosin in Methylated Calf Thymus DNA

  • Chae, Whi-Gun (Department of Biochemistry, School of medicien, Gatholic University of Taegu, Taegu 705-034)
  • Published : 2000.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Quantitation of N7-methyldeoxyguanosine (N7-MedG) produced in the in vitro N-methly-N-nitrosuourea (NMU) action on thymus DNA has been achieved by enzymatic degradation, liquid chromatoraphic separaphic separation and desorption chemical ionization tandem mass spectrometry. In conjunction with the resolving power HPLC in the separation of isomers, desorprion chemical ionization tandem mass spectrometry has utilized in determining modified nucleosides at low levels using a stable-isotope labled compound as an internal reforence. The quantitative estimation of N7-methyldeoxyguanosine was previously established by an independent HPLC analysis of methylated calf thymus DNA. A sensitive and specific methodogy for the quantitation of N7-MedG at the picomole level using HPLC combined with tandem mass spectrometry without radioisotope labeling process is presented. The potential of the liquid chromatoraphic tandem mass exposure to methlation agents in vitro.

Keywords

References

  1. Pharmacol. Therapeutics v.76 The mitomycin bioreductive antitumor agents: cross-linking and alkylation of DNA as the molecular basis of their activity Thomasz, M.;Y. Paslom
  2. Bioessays v.17 Alkylation if DNA and its aftermath Lawley, P. D.
  3. Mutation Research v.213 Mutagens as carcinogens: development of current concepts Lawley, P. D.
  4. Carcinogenesis v.1 A comprehensive quantitative analysis of methylated and ethylated DNA using high pressure liquid chromatography Beranek, D. T.;C. C. Weis;D. H. Swenson
  5. Bio. Mass Spectr. v.20 Chemical modification of DNA: quantitation of $O^6$-methyldeoxyguanosin by tandem mass spectrometry Chae, W.-G.;C.-J. Chang;J. M. Wood;R. G. Cooks
  6. J. Mass Spectr. Ion Processes v.111 Chemical modification of dexyribonucleic acids: quantitation of 3-methylthymidine and $O^4$-methylthynidin by tandem mass spctrometry Wood, J. M.;S. H. HokeⅡ;R. G. Cooks;W.-G. Chae;C.-J. Chang
  7. Prog. Nucleic Acid Res. Mol. Biol. v.15 The chemical effects of nucleic acid alkylation and their relation to mutagenesis and carcinogenesis Singer, B.
  8. Chem. Biol. Interact. v.70 Depurination from DNA of 7-methylguanine, 7-(20aminoethyl)-guanine and ring-opened 7-methylguanine Hemminki, K.;K. Peltonen;P. Vodicka
  9. Brit. Med. Bull. v.36 DNA as a target a alkylating carcinogens Lawley, P. D.
  10. Biochemistry v.20 Chemical modification of ribonucleic acid. A direct study by carbon-13 nuclear magnetic resonance spectroscopy Chang, C.-J.;C.-G. Lee
  11. Anal. Biochem. v.129 Reverse-phase high-performance liquid chromatography of chemically modified DNA Dasilva, G. J.;C.-J. Chang
  12. J. Chromatogr. v.455 Measurement of methylated DNA purines by reversed-phase high-performance liquid chromatography and fluorimetric detection Tacchi-Bedford, A. M.
  13. Carcinogenesis v.14 Quantification of 7-methyldeoxyguanosine using immunoaffinity Bianchini, F.;R. Montesano;D. E. G. Shuker;J. Cuzick;C. P. Wild
  14. Am. J. Respir. Cell Mol. Biol. v.15 Detection of $N^7$-Methyldeoxyguanosin Adducts in Human Pulmonary Alveolar Cells Petruzzelli, S.;L. M. Tavanti;A. Celi;C. Giuntini
  15. Chem. Biol. Interact. v.57 Modification of calf thymus DNA by methyl methanesulfonate. Quantotative determination of 7-methyldeoxyguanosin by mass spectrometry Chang, C.-J.;D. J. Ashworth;I. Isern-Flecha;X.-Y. Jiang;R. G. Cooks
  16. Biomed. Environ. Mass Spectrom v.14 Characterization of an Alkylated Dinucleotide by Desorption Chemical Iinizationand Tandem Mass Spectrometry Isern-Flecha, I.;X.-Y. Jiang;R. G. Cooks;W. Pfleiderer;W.-G. Chae;C.-J. Chang