Biotechnology and Bioprocess Engineering:BBE

  • 제5권2호
  • /
  • Pages.146-149
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  • 2000
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

High-efficient Expression of Porcine IL-2 with Recombinant Baculovirus Infected Silkworm, Bombyx mori

  • Inumaru, Shigeki (Department of Biological Product, National Institute of Animal Tsukuba) ;
  • KokuHo, Takehiro (Department of Biological Product, National Institute of Animal Tsukuba) ;
  • Yada, Takashi (Nikko Branch National Research Institure og Aquaculture, Nikko) ;
  • Kiuchi, Makoto (Department of Sericulture) ;
  • Miyazawa, Mitsusuhiro (Department of Insent Technology, National Insitute of Sericultural and Entomologcal Science)
  • 발행 : 2000.04.01
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초록

Biologically active porcine Interleukin-2(poIL-2) was produced from in vitro and in viva baculovirus expression system, namely the Autographa californica nuclear polyhedrosis virus (ACNPV)-cell culture system and the Hybrid nucler polthedrosis virus (HyNPV)-sillkworm larva system. The concentration of the recombinant poIL-2(rpoIL-2) in the larvae hemolymph was 1 to 3 mg/mL, which was about 7 to 20 times those of the cell culture systems. The level of this expression efficiency is equal to that with transgenic livestock, secretion products in milk.

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참고문헌

  1. The Cytokine Handbook(3rd ed.) Interleukin-2 and the Interleukin-2 Receptor Gaffen, S. L.;M. A. Goldsmith;W. C. Greene;A. Thomson(ed.)
  2. Bio/Technology v.6 Trends in the development of baculovirus expression vectors Luckow,V. A.;M. D. Summers
  3. Mol. Cell. Biol. v.3 Production of human beta interferon in insect cells infected with a baculovirus expression vector Smith, G. E.;M. D. Summers;M. J. Fraser
  4. Nature v.315 Production of human α-interferon in silkworm using a baculovirus vector Maeda, S.;T. Kawai;M. Obinata;H. Fujiwara;T. Horiuchi;Y. Saeki;Y. Sato;M. Furusawa
  5. J. Gen. Virol. v.73 Foreign gene expression by baculovirus vector with an expanded host range Mori, H.;H. Nakazawa;N. Shirai;N. Shibata;M. Sumida;F. Matsubara
  6. J. Gen. Virol. v.78 Efficient protein production using Bombyx mori nuclear poly-hedrosis virus lacking the cysteine proteinase gene Suzuki, T.;T. Kanaya;H. Okazaki;K. Ogawa;A. Usami;H. Watanabe;K. Kadono-Okuda;M. Yamakawa;H. Sato;H. Mori;S. Takahashi;K. Oda
  7. Biochim. Biophys. Acta v.1089 cDNA cloning of porcine interleukin 2 by polymerase chain reaction Goodall J. C.;D. C. Emery;L. S. English;L. Hall
  8. J. Gen. Virol. v.68 Baculovirus expression vectors: the requirements for high level expression of proteins, including glycoproteins Matsuura, Y.;R. D. Possee;H. A. Overton;D. H. L. Bishop
  9. Immunol. Cell Biol. v.76 Expression of biologically active recombinant porcine GM-CSF by baculovirus gene expression system Inumaru, S.;T. Kokuho;S. Denham;M. S. Denyer;E. Momotani;S. Kitamura;A. Corteyn;S. Brookes;R. M. E. Parkhouse
  10. Nucl. Acid Res. v.18 Linearization of baculovirus DNA enhances the recovery of recombinant virus expression vectors Kitts, P. A.;M. D. Ayres;R. D. Possee
  11. Virology v.152 Replication of the Tricoplusia ni granulosis and nuclear polyhedrosis viruses in cell cultures Granados, R. R.;C. G. Derksen;K. G. Dwyer
  12. Invertebrate Cell System Applications Gene transfer vectors of a baculovirus, Bombyx mori, and their use for expression of foreign genes in insect cells Maeda, S.;J. Mitsuhashi(ed.)
  13. Anal. Biochem. v.166 Tricine-sodium dodecyl sulfate-polyacrylamide gel electrophoresis for the separation of proteins in the range from 1 to 100kDa Stagger, H.;G. V. Jag
  14. Bull. Natl. Inst. Anim. Health MTT-DMSO assay: An improved colorimetric assay for the evaluation of cellular and immune function Takamatsu, H.;H. Murata