Microbiology and Biotechnology Letters (한국미생물·생명공학회지)

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Analysis and cloning of the gene involved in activation of maltose metabolism in Serratia marcescens.

Serratia marecscens에서 maltose 대사를 촉진하는 유전자의 클로닝 해석

  • 이승진 (동아대학교 생명자원과학부) ;
  • 유주순 (동아대학교 생명자원과학부) ;
  • 김혜선 (동아대학교 생명자원과학부) ;
  • 이상철 (동아대학교 생명자원과학부) ;
  • 정수열 (동주대학 식품영양과) ;
  • 최용락 (동아대학교 생명자원과학부)
  • Published : 2000.02.01
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Abstract

We have got several clones from Serratia marcescens which stimulated the cells to use maltose as a carbon source in Escherichia. coli TP2139 ( lac, crp). One of the cloned genes, pCKB17, was further analyzed. In order to find whether the increased expression of the gent was under the direction of maltose metabolism, we constructed several recombinant subclones. We have found that the clone, pCKB17AV, codes maltose metabolism stimulation(mms) gene. E. coli transformed with the cloned gene showed increase in the activity of maltose utilzation, The recombinant proteins expressed by multicopy and induction with IPTG, one polypeptide of 29-kDa, was confirmed by SDS-PAGE. The overexpression of maltose-binding proter protein in the presence of mms gene was confirmed by Western blot analysis. Southern hybridization analysis confirmed that the cloned DNA fragment was originated from S. marcescens chromosomal DNA.

Keywords

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