Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Effects of Mulberry (Morus alba L.) Leaf Extract on Oxygen Radicals and Their Scavenger Enzymes in Brain of SD Rats

뇌조직의 활성산소 및 그 제거효소에 미치는 뽕잎 추출물의 영향

  • 최진호 (부경대학교 식품생명공학부 생화학교실) ;
  • 김대익 (부경대학교 식품생명공학부 생화학교실) ;
  • 박수현 (부경대학교 식품생명공학부 생화학교실) ;
  • 김정민 (부경대학교 식품생명공학부 생화학교실) ;
  • 김창목 (한국산업정보기술연구원 생화학실) ;
  • 이희삼 (농촌진흥청 농업과학기술원 잠사곤충부) ;
  • 류강선 (농촌진흥청 농업과학기술원 잠사곤충부)
  • Published : 2000.12.01
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Abstract

This study was designed to investigate the effects of mulberry (Morus alba L.) leaf extract (MLE) on oxygen radicals and their scavenger enzymes in brain membranes of rats. Sprague-Dawley (SD) male rats (160$\pm$10 g) were fed basic diet (control group), and experimental diets (MLE-100 and MLE-300 groups) added 100 and 300 mg/kg BW/day for 6 weeks. Hydroxyl radical (.OH) lecels resulted in significant decreases (13.4% and 21.1%, 12.0% and 13.4%, respectively) in brain mitochondria and microsome of MLE-100 and MLE-300 groups compared with control group. Superoxide radical ($O_2$) levels were significantly decreased about 12% in brain cytosol of MLE-300 group compared with control group. Lipid peroxide (LPO) levels were effectively inhibited (18.1% and 12.3%, respectively) in brain mitochondria and microsomes of MLE-300 groups compared with control group. Oxidized protein (OP) levels were significantly decreased (14.2%, and 10.9%, respectively) in brain mitochondria and microsomes of MLE-300 groups compared with control group. Mn-SOD activities in brain mitochondria were significantly increased (13.5% and 18.6%, respectively) in MLE-100 and MLE-300 groups, and Cu/Zn-SOD activities in brain cytosol were also effectively increased (about 17.7%) in MLE-300 groups compared with control group. GSHPx activities in brain cytosol were remarkably increased (17.2% and 23.9%, respectively) in MLE-100 and MLE-300 groups compared with control group. These results suggest that anti-aging effect of mulberry leaf extract (MLE) may play a pivotal role in attenuating a various age-related changes in brain.

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