Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
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Production of Tetrodotoxin Using Novel Marine Microorganism Isolated from Intestine of Pufferfish

복어 장내에서 분리한 신규 해양 미생물을 이용한 Tetrodotoxin 생산

  • 윤성준 (경성대학교 공과대학 식품공학과) ;
  • 송성광 (경성대학교 공과대학 식품공학과) ;
  • 이명자 (서울식품의약품안전청 시험분석실) ;
  • 정동윤 (서울식품의약품안전청 시험분석실) ;
  • 김희숙 (경성대학교 공과대학 식품공학과) ;
  • 김동수 (경성대학교 공과대학 식품공학과) ;
  • 이은열 (경성대학교 공과대학 식품공학과)
  • Published : 2000.04.01
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Abstract

The production of tetrodotoxin (TTX) using Vibrio sp. YE-101, a novel marine microorganism isolated from the intestine of pufferfish, was investigated. Culture condition was optimized for the enhanced production of TTX using response surface methodology. The experimental sets of environmental conditions including pH, temperature and NaCl concentration were designed using central composite experimental design. The optimal conditions of pH, temperature and NaCl concentration were determined to be 8.1, 29.2℃, and 2.6% (w/v) respectively. The relative growth extent could be enhanced up to 80%, and final mouse unit (MU) value of TTX was also enhanced up to 87% by response surface optimization.

Keywords

References

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