Abstract
This study carried out to investigate activities of the superoxide dismutase(SOD), peroxidase(POD) and diterpene cyclase of rice plant treated by environmental stress conditions. The rice leaves of Ilpum was shown to have the most highest specific activity of POD. POD activity in the susceptible cv. Ilpum and Hwasung during the disease progress show a increase, while for a decreasing activity in the resistant cv. Dasan. SOD activity of rice plants treated by chitosan were increased as the treatment concentration increased. At the chitosan application, the SOD activity in the susceptible cv. Hwansung at the rice leaves 14days after treatment was higher in treatment than chitosan-untreated control. The activity of POD in the susceptible cv. Ilpum decreased as long as UV-B irradiation, while that in the resistant cv. Dasan increased. Futhermore, activity of that in Dasan were similar to as time on UV irradiation, Hwasung showed two times higher than untreated control at 40 minutes after UV-B exposure. The activity of diterpene cyclase in rice plants inoculated with Pyricularia oryzae has much higher tendency than UV-B irradiation. The activity of diterpene cyclase in the rice leaves gradually increased until 5 days after inoculation with Pyricularia oryzae.
UV-B와 도열병병원균 접종들의 환경 스트레스에 의한 벼의 POD, SOD및 Diterpene cyclase등의 활성 변화를 조사하여 해 본 결과, 일품벼에서 POD 와 SOD활성이 가장 놓았고, 도열병진전에 따른 PDO활성은 감수성 품종인 일품과 화성은 증가하였으나 저항성 품종인 다산은 감소하였다. 키토산 처리에의한 SOD의 활성 변화는 처리 농도가 높을수록 증가하였다. 키토산 처리후 14일에서의 감수성품종인 화성의 SOD활성은 무처리 식물체에 비하여 높았다. UV-B조사 시간이 길수록 감수성품종인 일폼의 POD활성은 감소하였고, 저항성 품종인 다산은 증가하였다. 특히UV-B의 조사시간에 따른 다산의 POD활성은 차가 없었으나, 화성의 활성은 40분 조사처리시 2배 놓았다 UV-B처리에 비해 p.oryzae를 접종시는 diterpene cyclase의 활성이 놓아졌으며, 병원균 접종후 5일까지 효소활성이 검진적으로 증가하였다.
