Journal of Life Science (생명과학회지)

Korean Society of Life Science (한국생명과학회)
권호 표지

Isolation of Urease Positive Vibrio parahaemolyticus and Urease Production

Urease를 생산하는 Vibrio parahaemolyticus의 분리 및 urease 생산

  • 김종숙 (동의대학교 미생물학과) ;
  • 김영희 (동의대학교 미생물학과)
  • Published : 2000.02.01
Download PDF
⟨ Previous Next ⟩

Abstract

Urease is an important microbial enzyme and its production is a marker to predict potential pathogenicity. An unusual halophilic bacterium producing urease was isolated from sea product and identified as Vibrio parahaemolyticus KH410. Its biochemical properties were indole negative, gelatin positive, sodium citrate positive and Kanagawa positive whereas other characteristics were identical as the standard strain except it showed a positive reaction on Christensen's urea agar. V. parahaemolyticus urease production was directly related to urea concentration. The production of urease was noticeable by the addition of 0.2% urea, 0.5% glucose, 2% NaCl in LB broth, and the initial pH of 5.5. The maximum production reached after 6 hr of incubation at 37$^{\circ}C$. However, NiCl2, metal ions, phosphorus did not affect production of urease.

Keywords

References

  1. J. Clin. Pathol. v.41 Cytopathic effects of Campylobacter pylori urease Barer, M. R.;T. J. Elliott;D. Berkeley
  2. J. Clin. laboratory Analysis v.10 Purification, characterization, and pathologenicity of urease produced by Vibrio parahaemolyticus Cai, Y.;N. Yuxing
  3. J. Infec. Dis. v.132 no.5 Cytotoxicity of Vibrio parahaemolyticus in Hela cell culture Carrthers, M. M.
  4. J. BIol. Chem. v.265 Purification and characterization of urease from Helicobacter pylori Dunn, B. E.;G. P. Campbell;G. I. Perez-perez;J. H. Baron
  5. J. Bacteriol. v.93 Influence of urea on the growth of T-strain mycoplasma Ford, D. K.;J. Macdonald
  6. Eur. J. Epidemiology v.8 no.6 A survey of urease-positive Vibrio parahaemolyticus strains isolated from traveller's diarrhea, sea water, and imported frozen sea foods Honda, S.;S. Matsumoto;T. Miwatani;T. Honda
  7. Rev. Med. Microbiol. v.4 The pathogenicity of Vibrio parahaemolyticus and the role of thermostable direct hemolysin and related hemolysins Honda, T.;T. Iida
  8. Appl. Environ. Microbiol. v.60 no.8 Urea hydrolysis can predict the potential pathogenicity of Vibrio parahaemolyticus strains isolated in the Pacific Northwest Kaysner, C. A.
  9. J. Bacteriol. v.132 Effect of urea concentration on growth of Ureaplasma urealyticum(T-strain mycoplasma) Kenny, G. E.;F. D. Cartwright
  10. J. Bacteriol. v.68 Purification and properties of bacterial urease Larsen, A. O.;R. E. Kallio
  11. J. Biol. Chem. v.193 Protein measurement with the Folin phenol reagent Lowry, O. H.;N. J. Rosebrough;A. L. Farr;R. J. Randall
  12. J. Bacteriol. v.125 Urea hydrolyzing activity of a T-strain mycoplasma: Ureaplasma urealyticum Masover, G. K.;J. E. Sawyer;L. H. Hatflick
  13. Vibrio parahaemolyticus Miwatani, T.
  14. Jpn. J. Med. Sci. Biol. v.16 Studies on the enteropathogenic facultatlvely, halophilic bacteria Vibrio parahaemolyticus.Ⅰ. Morphological, cultural and biochemical properties and its taxonomical position Sakazaki, R.;S. Iwanami;H. Fukumi
  15. Analytical chemistry v.39 Phenol hypochlorite reaction for determination of ammonia Weatherburn, M. W.