Conversion of Xylose to Ethanol by Recombinant Saccharomyces cerevisiae Containing Genes for Xylose Reductase and Xylose Reductase and xylitol Dehydrogenase from Pichia stipitis

  • Jin, Young-Su (Department of Food Science and Technology, Research Center for New Biomaterials in Agriculture, Seoul National University) ;
  • Lee, Tae-Hee (Department of Food Science and Technology, Research Center for New Biomaterials in Agriculture, Seoul National University) ;
  • Choi, Yang-Do (Division of Applied Biochemistry, Seoul National University) ;
  • Ryu, Yeon-Woo (Department of Molecular Science and Technology, Ajou University) ;
  • Seo, Jin-Ho (Department of Food Science and Technology, Research Center for New Biomaterials in Agriculture, Seoul National University)
  • Published : 2000.08.01

Abstract

A recombinant Saccharomyces cerevisiae, transformed with the genes encoding xylose reductase (XYL1) and xylitol dehydrogenase (XYL2) orginated from Pichia stipitis CBS 5776, was developed to directly convert xylose to ethanol. A fed-batch fermentation with the recombinant yeast produced 8.7 g ethanol/l with a yield of 0.13 g ethanol/g xylose consumed.

Keywords

References

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