Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Hormonal Effects of Several Chemicals in Recombinant Yeast, MCF-7 Cells and Uterotrophic Assays in Mice

  • Park, Jin-Sung (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Lee, Beom-Jun (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Kang, Kyung-Sun (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Tai, Joo-Ho (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Cho, Jae-Jin (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University) ;
  • Cho, Myung-Haing (Department of Toxicology, College of Veterinary Medicine, Seoul National University) ;
  • Inoue, Tohru (Division of Celular and Molecular Toxicology, National Institute of Health Science) ;
  • Lee, Yong-Soon (Department of Veterinary Public Health, College of Veterinary Medicine, Seoul National University)
  • Published : 2000.06.01
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Abstract

Many methods have been developed for screening chemicals with hormonal activity. Using recombinant yeasts expressing either human estrogen receptor [Saccharomyces cerevisiae ER + LYS 8127 (YER)] or androgen receptor [S. cerevisiae AR + 8320 (YAR)], we evaluated the hormonal activities of several chemicals by induction of ${\beta}-galactosidase$ activity. The chemicals were $17{\beta}-estradiol$ (E2), testosterone (T), ${\rho}-nonylphenol$ (NP), bisphenol A (BPA), genistein (GEN), 2-bromopropane (2-BP), dibutyl phthalate (DBP), di-(2-ethylhexyl) phthalate (DEHP), 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), and butylparaben (BP). To assess the estrogenicity of NP, the result of the in vitro recombinant yeast assay was compared with an E-screen assay using MCF-7 human breast cancer cells and an uterotrophid assay using ovariectomized mice. In the YER yeast cells, E2, NP, BPA, GEN, and BP exhibited estrogenicity in a doseresponse manner, while TCDD did not. All the chemicals tested, except T, did not show androgenicity in the YAR yeast cell. The sensitivity of the yeast (YER) assay system to the estrogenic effect of NP was similar to that of the E-screen assay. NP was also estrogenic in the uterotrophic assay. However, in terms of convenience and costs, the yeast assay was superior to the E-screen assay or uterotrophic assay. These results suggest that the recombinant yeast assay can be used as a rapid tool for detecting chemicals with hormonal activities.

Keywords

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