Genetic Analysis of absR, a new abs locus of Streptomyces coelicolor

  • 발행 : 2000.04.01

초록

The filamentous soil bacterium Streptomyces coelicolor is known to produce four distinct antibiotics. The simultaneous global regulation for the biosynthesis of those four antibiotics was previously confirmed by absA and absB mutations that blocked all four antibiotics' biosynthesis without influencing their morphological differentiation. To study the complex regulatory cascade that controls the secondary metabolism in Streptomyces, a new abs-like mutation was characterized. namely absR, which is slightly leaky on a complete R2YE medium, yet tight on a minimal medium. A genetic analysis of the absR locus indicated that it is located at 10 o'clock on the genetic map, near the site of absA. A cloned copy of the absA gene that encoded bacterial two-component regulatory kinases did not restore antibiotic biosyntheis to the absR mutant. Accordingly, it is proposed that absR is another abs-type mutation which is less tight than the previously identified absA or absB mutations income medium conditions, and can be used to characterize another global regulatory gene for secondary metabolete formation in S. coelicolor.

키워드

참고문헌

  1. J. Bacteriol. v.180 Transcriptional regulation of Streptomyces coelicolor pathway specific antibiotic regulators by the absA and absB loci Aceti, D. J.;W. C. Champness
  2. J. Bacteriol. v.174 Genetic analysis of absB: A Streptomyces coelicolor locus involved in global antibiotic regulation Admidis, T.;W. Champness
  3. J. Bacteriol. v.172 Mutations in a new Streptomuyces coelicolor locus which globally block antibiotic biosynthesis but not sporulation Adamidis, T.;P. Riggle;W. Champness
  4. J. Bacteriol. v.178 Global negative regulation of Streptomyces coelicolor antibiotic synthesis mediated by an absA-encoded putative signal transduction system Brian, P.;P. J. Riggle;R. A. Santos;W. C. Champness
  5. Gene v.115 Identification of Streptomyces coelicolor genes involved in regulation of antibiotic synthesis Champness, W.
  6. J. Bacteriol. v.107 New loci required for Streptomyces coelicolor morphological and phsiological differentiation Champness, W. C.
  7. Regulation of Bacterial Differentiation Regulation of antibiotic production and morphological differentiation on Streptomyces spp. Champness, W. C.;K. F. Chater;P. Piggot(ed.);C. P. Moran(ed.);P. Youngman(ed.)
  8. Products of Secondary Metabolism. Biotechnology Regulation of bacterial antibiotics production Chater, K. F.;M. J. Bibb;H. Kleinkauf(ed.);H. von Dohren(ed.)
  9. Gene v.26 Mutational cloning in Streptomyces and the isolation of antibiotic production genes Chater, K. F.;C. J. Bruton
  10. Mol. Microbiol. v.4 Spore colour in Streptomyces coelicolor A3(2) involoves the developmentally regulated synthesis of a compound biosynthetically related to polyketide antibiotics Davis, N. K.;K. F. Chater
  11. J. Gen. Microbiol. v.131 Genetic and biochemical characterization of the red gene cluster of Streptomyces coelicolor A3(2) Feitelson, J.;F. Malpartida;D. A. Hopwwod
  12. Cell v.66 The act cluster contatins regulatory and antibiotic export genes, direct target for translational control by the bldA tRNA gene of Streptomyces Fernandes-Moreno, M. A.;J. L. Caballero;D. A. Hopwood
  13. J. Bacteriol. v.174 abaA, a new pleiotropic regulatory locus for antibiotic production in Streptomyces coelicolor Fernandes-Moreno, M. A.;A. J. Martin-Triana;E. Martinez;J. Niemi;H. M. Keiser;D. A. Hopwood;F. Malpartida
  14. J. Microbiol. Biotechnol. v.8 Effects of protein kinase inhibitors on in vitro protein phosphorylation and on secondary metabolism and morphogenesis in Streptomyces coelicolor A3(2) Hong, S. K.;S. Horinouchi
  15. J. Bacteriol. v.173 Phosphorylation of the AfsR product, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2) Hong, S. K.;M. Kito;T. Beppu;S. Horinouchi
  16. Mol. Gen. Genet. v.236 Effects of protein kinase inhibitors on in vitro protein phosphorylation and cellular differentiation of Streptomyces griseus Hong, S. K.;A. Matsumoto;S. Horinouchi;T. Beppu
  17. Genetic Manipulation of Streptomyces: A Laboratory Manual Hopwood, D. A.;M. J. Bibb;K. F. Chater;T. Kieser;C. J. Bruton;H. M. Kewser;D. J. Lydiate;C. P. Smith;J. M. Ward;H. Schrempf
  18. J. Bacteriol. v.129 CDA is a new chromosomally-determined antibiotic from Streptomyces coelicolor A3(2) Hopwood, D. A.;H. M. Wright
  19. Gene v.115 Regulation of secondary metabolism and cell differentiation in Streptomyces: A-factor as a microbial hormone and the AfsR protein as a component of a two-component regulatory system Horinouchi, S.;T. Beppu
  20. J. Bacteriol. v.155 Cloning of a pleiotropic gene that positively controls biosynthesis of A-factor, actinorhodin, and prodigiosin in Streptomyces coelicolor A3(2) and Streptomyces lividans Horinouchi, S.;O. Hara;T. Beppu
  21. Gene v.95 Primary structure of AfsR, a global regulatory protein for secondary metabolite formation in Streptomyces coelicolor A3(2) Horinouchi, S.;M. Kito;M. Nishiyama;K. Furuya;S. K. Hong;K. Miyake;T. Beppu
  22. J. Bacteriol. v.174 A putative two-component regulatory system involved in secondary metabolism in Streptomyces spp. Ishizuka, H.;S. Horinouchi;H. M. Kieser;D. A. Hopwood;T. Beppu
  23. J. Gen. Microbiol. v.129 Cloning and expression of the tyrosinase gene from Streptomyces antibioticus in Streptomyces lividans Katz, E.;C. Thompson;D. Hopwood
  24. J. Gen. Microbiol. v.98 Genetic determination of methylenomycin synthesis by the SCP1 plasmid of Streptomyces coelicolor A3(2) Kirby, R.;D. Hopwood
  25. J. Microbiol. Biotechnol. v.8 Purification and characterization of Streptomyces griseus trypsin overexpressed in Streptomyces lividans Koo, B.-N.;K.-H. Bai;S. M. Byun;S.-K. Hong
  26. Proc. Natl. Acad. Sci. USA v.88 TTA codons in some genes prevent their expression in a class of developmental, antibiotic-negative Streptomyces mutants Leskiw, B. K.;E. J. Lawlor;J. M. Fernandz-Abalos;K. F. Chater
  27. J. Virol. v.9 Characterization of temperate actinophage φC31 isolated from Streptomyces coelicolor A3(2) Lomovskya, N. D.;N. M. Mjrtumian;N. L. Gostimskaya;V. N. Danilenko
  28. Nature v.309 Molecular cloning of the whole bisynthetic pathway of a Streptomyces antibiotic and its expression in a heterologous host Malpartida, F.;D. A. Hopwood
  29. Mol. Gen. Genet. v.205 Physical and genetic characterisation of the gene cluster for the antibiotic actinorhodin in Streptomyces coelicolor A3(2) Malpartida, F.;D. A. Hopwood
  30. Gene v.93 Cloning and expression in a heterologous host of the complete set of genes for biosynthesis of the Streptomyces coelicolor antibiotic undecylprodigionsin Malpartida, F.;J. Niemi;R. Navarrete;D. A. Hopwood
  31. Gene v.146 Phosphorylation of the AfsR protein involved in secondary metabolism in Streptomyces species by a eukaryotic-type protein kinase Matsumoto, A.;S. K. Hong;H. Ishizuka;S. Horinouchi;T. Beppu
  32. J. Gen. Microbiol. v.96 A morphological and genetic mapping study of bald colony mutants of Streptomyces coelicolor Merrick, M. J.
  33. Actinomycetologica v.12 Regulatory factors in physiological differentiation of Streptomyces coelicolor Park, E.;S.-K. Hong
  34. FEMS Microbiol. Lett. v.144 Expression of the Streptomyces coelicolor A3(2) ptpA gene encoding a phosphotyrosine protein phosphatase leads to overproduction of secondary metabolites in Strepomyces lividans Umeyama, T.;Y. Tanabe;B. D. Aigle;S. Horinouchi
  35. J. Gen. Microbiol. v.64 Genetic control of fertility in Streptomyces coelicolor A3(2): The IF fertility type Vivian, A.;D. A. Hopwood