Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
권호 표지

Comparative Evaluation of Probiotic Activities of Bifidobacterium longum MK-G7 with Commercial Bifidobacteria Strains

  • Jung, Hoo-Kil (R&D, Central Research Institute, Maeil Dairy Industry Co., Ltd.) ;
  • Kim, Eung-Ryool (R&D, Central Research Institute, Maeil Dairy Industry Co., Ltd.) ;
  • Ji, Geun-Eog (Department of food Science and Nutrition, Seoul National University) ;
  • Park, Jong-Hyun (Department of Food and Bioengineering, Kyungwon Universisy) ;
  • Cha, Seong-Kwan (Korea Food Research Institute) ;
  • Juhn, Suk-Lak (R&D, Central Research Institute, Maeil Dairy Industry Co., Ltd.)
  • Published : 2000.04.01
Download PDF
⟨ Previous Next ⟩

Abstract

This study was conducted to compare probiotic activities and physiological functions of Bifidobacterium longum Mk-G7 with weveral commercial and type strains of bifidobacteria. bif. longum MK-G7 showed the highest acid tolerance against HCl and acetic acid, whereas bif. infantis Y-1 showed the lowest acid tolerance and more than 4 log cycles of viable cell count decreased due to acid injuty. Viable cell counts of bifidobacteria strains decreased more than 1.5 log cycles owing to oxygen toxicity, with the exception of Bif. longum MK-G7, Bif. infantis Y-2, Bif. longum Y-3, Bif. longum Y-6, and Bif. longum RD-13 showed the highest bile tolerance, whereas Bif. longum MK-G7 showed a medium level of bile tolerance. Only Bif. longum MK-G7 howed much higher antibiotic resistance against both tetracycline and penicillin-G in the MIC(minimum inhibitory concentration) level of 24.8 mg/I and 0.52mg/I, respectively. Bif longum Y-6, and Bif. bifidum ATCC 29539 showed more than 80% of anti-mutagenicity against NQO(4-nitroquinolinel-oxide). Since the production of cytokines such as $TNF(tumor necrosis factor)-{\alpha}$ and IL (interleukin)-6, and NO(nitric oxide) in the macrophage cell line Raw 264.7 cells increased as Bif. longum MK-G7 cell concentration increased, ti was suggested that Bif. longum MK-G7 is able to enhance immunopotentiating activity in vitro. When freeze-dred Bif. longum MK-G7 was administered to mice at the dose of 1,2,4, and 6 g/kg of body weight, all of the mice survived in all feeding groups, proving the GRAS(generally recognized as safe) status of Bif. longum MK-G7. When fermented milk containing Bif. longum MK-G7 was administered to human volunteers, viable cell count of total bifidobacteria and anaerobes in the feces increased up to 0.5 log cycles more than before the administration. In particular, Bif. logum MK-G7 ingibited the growth of Bacteroides at the level of 1.0-1.5 log cycles.

Keywords

References

  1. Lactic Acid Bacteria Bifidobacteria and probiotic action Ballongue, J.;Salminen, S.(ed.);A. von Wright(ed.)
  2. Microbiol. Inmunol. v.36 Impact of Bifidobacterium longum on human fecal miroflora Benno, T.;T. Mitsuoka
  3. Gastroenterology v.102 Fecal recovery in humans of viable Bifidovacterium sp. ingested in fermented milk Bouhnik, Y.;P. Pochart;P. Marteau;G. Arlet;I. Goderel;J. C. Rambaud
  4. J. Dairy Res. v.61 Inhibitory effect of dairy products on the mutagenicties of chemicals and dietary mutagens Cassand, P.;H. Abdelali;C. Bouley;G. Denariaz;J. F. Narbonne
  5. Int. J. Food Microbiol. v.47 Screenig and selection of acid and bile resistant bifidobacteria Chung, H. S.;Y. B. Kim;S. L. Juhn;G. E. Ji
  6. Cultured Dairy Products J. v.28 Selection of bifidobacteria for use as dietary adjuncts in cultured dairy foods. Ⅱ. Tolerance to simulated pH of human stomachs Clark, P. A.;L. N. Cotton;J. H. Martin
  7. J. Anim. Sci. v.67 Anticholesteremic property of Lactobacillus acidophilus yogurt fed to mature boars Danielson, A. D.;E. R. Peo;K. M. Shahani;A. J. Lewis;P. J. Whalen;M. A. Amer
  8. Eur. J. Clin. Nutr. v.45 Probitics and stimulation of the immune response De Simone, C.;E. Rosati;S. Moretti;B. Bianchi-Salvadori;R. Vesely;E. Jirillo
  9. Biochim. Biophys. Acta v.136 Carbohydrate metabolism in Bifidobacterium bifidum De Vries, W.;S. J. Gerbrandy;A. H. Stouthamer
  10. J. Bacteriol. v.93 Pathway of glucose fermentation in relation to the taxonomy of bifidobacteria De Vries, W.;A. H. Stouthamer
  11. J. Bacteriol. v.96 Fermentation of glucose, lactose, galactose, mannitol, and xylose by bifidobacteria De Vries, W.;A. H. Stouthamer
  12. Toxicol. Appl. Pharmacol. v.127 Elevated gene expression and production of interleukins 2, 4, 5, 6 during exposure to vomitoxin (deoxyivalenol) and cycloheximide in the EL-4 thymoma Dong, W.;J. I. Azcona-Olivera;K. H. Brooks;J. E. Linz;J. J. Pestka
  13. Asia Pacific J. Clin. Nutr. v.5 Safety of probiotic bacteria Donahue, D. C.;S. Salminen
  14. Neth. Milk Dairy J. v.43 Fermented milks with selected intestinal bacteria: A healthy trend in new products Driessen, F. M.;R. De Boer
  15. Milchwissenschaft v.47 The peptide hydrolase system of Bifidobacterium species El-Soda, M.;A. Macedo;N. F. Olsen
  16. J. Appl. Bacteriol. v.66 Probiotics in man and animals Fuller, R.
  17. Gut v.32 Probiotics in human medicine Fuller, R.
  18. Lett. Appl. Microbiol. v.19 α-Galactosidase (EC 3.2.1.22) from Bifidobacterium longum Garro, M. S.;G. S. de Giori;G. F. de Valdez;G. Oliver
  19. J. Dairy Sci. v.67 Importance of bile tolerance of Lactobacillus acidophilus used as a dietary adjunct Gilliland, S. E.;T. E. Staley;L. J. Bush
  20. FEMS Microbiol. Rev. v.87 Health and nutritional benefits from lactic acid bacteria Gilliland, S. E.
  21. Am. J. Clin. Nutr. v.32 Hypocholesterolemic effect of yogurt and milk Hepner, G.;R. Fried;St. Jeor;L. Fusetti;R. Morin
  22. Am. J. Clin. Nutr. v.32 Hypocholesterlemic effect of yogurt and milk Hoover, G.;R. Fried;St. Jeor;L. Fusetti;R. Morin
  23. J. Sci. Food Agr. v.62 Survival of bifidobacteria in the presence of bile salt Ibrahim, S. A.;A. Bezkorovainy
  24. Kor. J. Food Sci. v.25 Improved selective medium for isolation and enumeration of Bifidobacterium sp. Ji, G. E.;S. K. Lee;I. H. Kim
  25. Korean J. Appl. Microbiol. Biotechnol. v.22 Composition and distribution of interstinal microbial flora in Korean Ji, G. E.
  26. Korea Patent No. 98-26354 Bifidobacterium longun MK-G7 strains and its applications Juhn, S. L.;H. K. Jung;E. R. Kim;G. E. Ji;J. H. Park
  27. Cultured Dairy Products J. v.25 Bifidobacteria as possible dietary adjuncts in cultured dairy products - A review Laroia, S.;J. H. Martin
  28. Cultured Dairy Products J. v.26 Effect of pH on survival of Bifidobacterium bifdum and Lactobacillus acidophilus in frozen fermented dairy desserts Laroia, S.;J. H. Martin
  29. FEMS Immunol. Med. Microbiol. v.10 Modulation of a specific humoral immune response and changes in intestinal flora mediated through fermented milk intake Link-Amster, H.;F. Rochat;K. Y. Sandan;O. Mignot;J. M. Aeschimann
  30. Atherosclerosis v.26 A factor in yogurt which lowers cholesterolemia in man Mann, G. V.
  31. Mutation Res. v.113 Revised methods for the Salmonella mutagenicity test Maron, D. M.;B. N. Ames
  32. J. Dairy Sci. v.80 Survival of lactic acid bacteria in a dynamic model of the stomach and the small intestine. Validation and the effect of bile. Marteau, P.;M. Minekus;R. Havenaar;J. H. J. Huis In't Veld
  33. Cultured Dairy Products J.26 v.27 Selection of bifidobacteria for use as dietary adjuncts in cultured dairy foods. I. Tolerance to pH of yogurt Martin, J. H.;K. M. Chou
  34. J. Clin. Microbiol. v.6 Bifidobacteria and their role in human health Misuoka, T.
  35. Biosci. Biotech. Biochem. v.60 Purification and characterization of β-D-glucosidase(β-D-fucosidase) from Bifidobacterium breve clb acclimated to cellobiose Nunoura, N.;K. Ohdan;T. Yono;K. Yamamoto;H. Kumagai
  36. J. Microbiol. Biotechnol. v.9 Comparison of nitric oxide, hydrogen peroxide, and cytokine production in RAW 264.7 cells by Bifidobacterium and other intestinal bacteria Om, A. S.;S. Y. Park;I. K. Hwang;G. E. Ji
  37. Int. J. Food Microbiol. v.46 Potentiation of hydrogen peroxide, nitric oxide, and cytokine production in RAW 264.7 macrophage cells exposed to human and commercial isolates of Bifidobacterium Park, S. Y.;G. E. Ji;Y. T. Ko;H. K. Jung;Z. Ustunol;J. J. Pestka
  38. Nutr. Res. v.15 Lactase activity of Bifidobacterium bifidum Passerat, B.;A. M. Desmaison
  39. Am. J. Clin. Nutr. v.55 Survival of bifidobacteria ingested via fermented milk during their passage through the human small intestine: An in vivo study using intestinal perfusion Pochart, P.;P. Marteau;Y. Bouhnik;I. Goderel;P. Bourlioux;J. C. Rambaud
  40. Bifidobacteria Microflora v.9 Bifidobacteria cultures as components of yoghurt like products Reuter, G.
  41. Milchwissenschaft v.45 Growth requirements of Bifidobacterium strains in milk Roy, D.;F. Dussault;P. Ward
  42. Appl. Microbiol. Biotechnol. v.34 Sugars fermented by Bifidobacterium infantis ATCC 27920 in relation to growth and α-galactosidase activity Roy, D.;P. Chevalier;P. Ward;L. Savoie
  43. Appl. Microbiol. Biotechnol. v.34 α- and β-ga;actpsodase activity Roy, D.;L. Blanchette;L. Savoie;P. Ward;P. Chevalier
  44. Ann. Microbiol. Enzymol. v.15 The fructose-6-phosphate shunt as a peculiar pattern of hexose degradation in the genus Bifidobacterium Scardovi, V.;L. D. Trovatelli
  45. J. Dairy Sci. v.78 Immunomodulation of human blood cells following the ingestion of lactic acid bacteria Schiffrin, E. J.;F. Rochat;H. Link-Amster;J. M. Aeschlimann;A. Donnet-Hughes
  46. Jpn. Pediatrics v.27 Action of intestinal microorganisms on humoral immunity in the digestive tract Takano, K.;M. Hasegawa;S. Tomizawa;K. Sakai
  47. Bifidobacteria Microflora v.5 Immunity provided by colonized enteric bacteria Ueda, K.
  48. Bifidobacteria Microflora v.10 Immune response of Bifidobacterium-monoassociated mice Yamazaki, S.;S. Tsuyuki;H. Akashiba;H. Kamimura;M. Kimura;T. Kawashima;K. Ueda