Korean Journal of Microbiology (미생물학회지)

The Microbiological Society of Korea (한국미생물학회)
권호 표지

Characterization of an Aniline-degrading Bacterium, Delftia sp. JK-2 Isolated from Activated Sludge of Municipal Sewage Treatment Plant

도시폐수처리장의 활성슬러지에서 분리한 Aniline 분해세균 Delftia sp. JK-2의 특성연구

  • Published : 2000.06.01
Download PDF
⟨ Previous Next ⟩

Abstract

Activated sludge samples were collected from a municipal sewage treatment plant and used for enrichment of microbial consortia with aniline as the sole carbon and nitrogen source. Threc aniline-degrading bacteria were obtained lrom microbial consortia and an isolate which has excellent aniline degradability was selected for this study. The isolate was Gram-negative, and identified and designated as Delfha sp. JK-2 on the basis of various physiological and biochemical tests. 10 mM aniline was completely degraded within 24 hours after inoculation of the culture. Ammonium ion was liberated in the medium transiently during the incubation and disappeared when aniline was completely degraded. Addition of glucose as a supplementary source to aniline minimal media showed significant decrease in aniline degradat~on rate for the strain Effective degradation of aniline was achieved by the addition of 0.5% nitrate as a nitrogen source, and resulted in approximately 80% higher aniline degradation compared to the absence of nitrate. Phylogenetic analysis based on 16s [DNA sequence revealed that the strain was closely related to De@ia acidovorans, with 96% overall similarity. The 16s [DNA sequence of JK-2 was also found to be closely related to those of six other clonal types, including Acidovoru, Aquaspirillum. Xylophilus, Variovorm, and Rhodofernr.

도시폐수처리장의 활성슬러지 표본으로부터 유일 탄소원 및 질소원으로 aniline을 이용할수 있는 미생물 컨소시엄을 농화배양하였다. 농화배양으로부터 분리된 3개의 분해세균 가운데 분해능이 탁원한 세균을 본 연구에 사용되었다. 분리세균운 그람 음성세균으로서 여러 가지 생리.생화학적 시험을 통하여 Delftia acidovorans로 동정되었으며 이를 Delftia sp. JK-2로 명명하였다. 10mM의 aniline이 포함된 액체배지에 Delftia sp. JK-2를 접종한 결과 24시간 이내에 aniline이 완전히 분해되엇다. 이 가간동안 배양액 내에 $NH_4^+$이온은 일시적으로 생성되었다가 aniline이 완전히 분해됨에 따라 완전히 사라졌다. Aniline을 포함하여 무기배지에 부가탄소로서 glucose를 첨가하였을 때 분해능은 크게 감소하였다. 질소원으로서 0.5% nitratef,f 첨가하였을 때 첨가하지 않은 대조군에 비해 anline의 분해가 80% 이상 향상되었다. Aniline 분해세균의 16S rDNA 염기서열을 이용하여 phylogenetic 분석을 실시한 결과 이 세균은 Delftia acidovorans와 96%의 유사성을 나타내었으며, Acidovorax, Aquaspirllum, Xylophilus, Variovorax, Rhodoferax 등의 세균과도 상당한 유사성을 나타내었다.

Keywords

References

  1. Chemical analysis of ecological materials. Inorganic constituents ; nitrogen Allens, S.E.;H.M. Grimshaw;J.A. Parkinson;C. Quarmby;S. E.Allen(ed.)
  2. Agric. Biol. Chem. v.47 Isolation of aniline-assimilating bacteria and physiological characterization of aniline biodegradation in Rhodococcus erythropolis AN-13 Aoki, K.;K. Ohtsuka;R. Shinke;H. Nishira
  3. Agric. Biol. Chem. v.47 Metabolism of aniline by Rhodococcus erythropolis AN-13. Aoki, K.;R. Shinke;H. Nishira
  4. J. Agric. Food Chem. v.26 Adsorption and transformation of four substituted aniline in soil. Bollag, J. M.;P. Blattman;T. Laanio
  5. Food Chem. Toxicol. v.28 Toxicity of p-chloroaniline in rats and mice Chabra, R.S.;M. Thompson;M.R. Elwell;A. C. Peters
  6. Microbiol. Rev. v.55 Biodegradation of halogenated organic compounds Chaudhry, G.R.;S.Chapalamadugh
  7. Standard methods for the examination of water and wastewater(14th ed.) Franson, M. A.
  8. Manual of methods for general bacterioloy. Gerhart, P.;R.G.E. Murray;R. N. Costillow;E. W. Nester;W. A. Wood;N. R. Kreig;G. B. Phillis
  9. FEMS Microbiol. Rev. v.103 Microbial breakdown of halogenated aromatic pesticides and related compound Haggblom, M. M.
  10. Ecotoxicol. Environ. Safety v.20 Influence of cytochrome P450 mixed function oxidase induction on the acute toxicity to rainbow trout fo primary aromatic amines Hermans, J.L.;S.P. Bradbury;S. J.Broderius
  11. Biochem. Mol. Biol. Intl. v.29 Oxidation of aniline to nitrobenzene be nonheme bromoperoxidase Itoh, N.;M. Naoki;K. Toyoko
  12. FEMS Microbiol Lett. Characterization of strain HY 99, a novel microorganism capable of aerobic and anaerobic degradation of aniline Kahng, H.Y.;J.J. Kukor;K. H. Oh
  13. J. Agric. Food Chem. v.17 Mixed chlorobenzene formation in soil Kearney, P.C.;J.R. Plimmer;F. B. Guardia
  14. Herbicides: chemistry, degradation, and mode of action(2nd ed.) Kearney, P.C.;D.D. Kaufman
  15. Arch. Microbiol. v.155 Degradation of aniline and monochlorinated anilines by soil-born Pseudomonas acidovorans strains Loidle, M.;C. Hinteregger;G. Ditzemuller;A. Ferschel;F. Streichsbier
  16. Microbial degradation of xenobiotics and recalcitranst compounds. Biodergadation of synthetic organic colorants Meyer, U.;T. Leisinger;R. Hutter;A.M. Cook;J. Nuesch(ed.)
  17. J. Agric. Food. Chem. v.25 Chemical transformation of 4-chloroaniline to a triazene in a bacterial culture medium Minard, R.D.;S. Russel;J.M. Bollag
  18. Mikro. Bioloya. v.49 Aniline as the sole source of carbon, nitrogen, and energy for Alcaligenes faecalis Surovtseva, E. G.;A. I. Bol'nova
  19. J. Toxicol. Environ. Health v.9 Mutagenic and toxic activity of environmental effluents from underground coal gasification experiments Timourian, H.;J. S. Felton;D. H. Stuermer;S. Healy;P. Berry;M. Tompkins;G. Battaglia;F. T. Hatch;L. H. Thompson;A. V. Carrano;J. Minkler;E. Salanzer
  20. Appl. Environ. Microbiol. v.51 Evolved aniline catabolism in Acinetobacter calcoaceticus during continuous culture of river water Wyndham, R. C.
  21. J. Aric. Food Chem. v.30 Metabolism of dichloroaniline by Pseudomonas putida You, I.S.;R. Barth
  22. Appl. Environ. Microbiol. v.50 Microbial mineralization of ring-substituted aniline through an ortho-cleav-age pathway Zeyer, J.;A. Wasserfallen;K. N. Timmis