Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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Construction of a Bioluminescent Reporter Using the luc Gene and meta-Cleavage Dioxygenase Promoter for Detection of Catecholic Compounds

  • Park, Sang-Ho (Department of Microbiology and Biotechnology, and Research Institute for Genetic Engineering, Chungbuk National University) ;
  • Lee, Dong-Hun (Department of Microbiology and Biotechnology, and Research Institute for Genetic Engineering, Chungbuk National University) ;
  • Oh, Kye-Heon (Department of Life Science, Soonchunhyang University) ;
  • Kim, Chi-Kyung (Department of Microbiology and Biotechnology, and Research Institute for Genetic Engineering, Chungbuk National University)
  • Published : 2000.09.01
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Abstract

Several types of bioluminescent reporter strains have been developed for the detection and monitoring of pollutant aromatics contaminating the environment. In this study, a bioluminescent reporter strain, E. coli SHP3, was constructed by fusing the luc gene of firefly luciferase with the promoter of pcbC responsible for the meta-cleavage of aromatic hydrocarbons. the bioluminescence expressed by the luc gene in the reporter was well triggered by the promoter when it was exposed to 2,3-dihydroxybiphenyI (2,3-DHBP) at 0.5 to 1 mM concentrations. The bioluminescent response was more extensive when the reporter strain was exposed to 5 mM catechol and 2 mM 4-chlorocatechol. These different types of bioluminescent responses by E. coli SHP3 appeared to be characterized by the nature of the aromatics to stress. Since E. coli SHP3 responded to 2,3-DHBP quite sensitively, this reporter strain could be applied for detecting some catecholic pollutants.

Keywords

References

  1. Appl. Environ. Microbiol v.64 A chromosomally based tod-luxCDABE whole-cell reporter for benzene, toluene, ethylbenzene, and xylene (BTEX) sensing Applegate B.M.;S.R. Kenrmeyer;G.S. Sayer
  2. Appl. Environ. Microbiol v.64 Identifi-cation and quantification of toxic chemicals by use of Escher-ichia coli carrying lux genes fused to stress promoters Ben-israel O.;H. Ben-israel;S. Ulizur
  3. Proc. Natl. Acad. Sci. v.80 Cloning of firefly luciferase cDNA and the expression of active luciferase in Escherichia coli deWet J.R.;K.W. Wood;M. De Luca
  4. Appl. Environ. Microbiol v.60 Optical biosensor for envi-ronmental on-line monitoring of naphthalene and salicylate bioavailability with an immobilized bioluminescent catabolic reporter bacterium Heitzer A.;K. Malachowsky;J.E. Thonnard;P.R. Bienkowski;D.C. White;G.S. Sayler
  5. Kor. J. Microbiol. (Presently J. Microbiol) v.32 Cloning and expression of pcbCD genes in Escherichia coli from Psedomonas sp. DJ-12 Kim C.K.;T.K. Sung;J.H. Nam;Y.C. Kim;J.K. Lee
  6. Appl. Environ. Microbiol v.62 Genetic structure of the gene encoding 2,3-DHBP dioxygenase and HOPDA hydrolase from biphenyl-and 4CB-degrading Pseudomonas sp. strain DJ-12 Kim E.;Y. Kim;C-K. Kim
  7. Science v.249 Rapid, sensitive bioluminescent reporter technology for naph-thalene exposure and biodegradation King J.M.H.;P.M. Digrazia;B. Applegate;R. Burlage;J. Sanseverino;P. Dunbar;F. Larimer;G.S. Sayler
  8. Appl. Environ. Microbiol v.64 Construction of bioluminescent repoter strain to detect polychlorinated biphenyls Layton A.C.;M. Muccini;M.M. Ghosh;G.S. Sayler
  9. Molecular cloning (2nd ed) Sambrook J.;E.F. Fritsch;T. Maniatis
  10. Appl. Environ. Microbiol v.59 Biolu-minescent sensors for detection of bioavailble Hg(II) in the environment Selifonova O.A.;R. Burlage;T. Barkay
  11. Appl. Environ. Microbiol v.62 Use of ipb-lux fusion to study regulation of the isopropylbenzene catabolism operon of Pseudomonas putida RE204 and to detection hydrophobic pollutants in environment Selifonova O.A.;R.W. Eaton
  12. Appl. Environ. Microbiol v.63 Recom-binant luminescent bacteria for measuring bioavailable arsenite and antimonite Taurianen S.;M. Karp;W. Chang;M. Virta
  13. Anal. Biochem v.272 Mea-surement of firefly luciferase reporter gene activity from cells and lysates using Escherichia coli arsenite and mercury sen-sors Tauriainen S.;M. Virta;W. Chang;M. Krap
  14. Appl. Environ. Microbiol v.60 Rapid and sensitive pol-lutant detection by induction of heat shock gene-biolumines-cence gene fusions Van Dyk T.K.;W.R. Najarian;K.B. Konstantinov;R.M. Young;P.S. Dhurjati;R.A. LaRossa
  15. J. Bacteriol v.177 Synergistic induction of the heat shock response in Escherichia coli by simultaneous treatment with chemical induces Van Dyk T.K.;T.R. Reed;A.C. Vollmer;R.A. LaRosa
  16. Appl. Environ. Microbiol v.64 Development and testing of a bacterial biosensor for toluene-based environmental con-taminants Willardson B.M.;J.F. Wilkins;T.A. Rand;J.M. Schupp;K.K. Hill;P. Keim;P.J. Jackson