Toxicological Research

  • 제16권1호
  • /
  • Pages.77-82
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  • 2000
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지

사람유래의 섬유아세포 배양을 이용하여 NRU(neutral red uptake) 시험법에 의한 in vitro 광독성 대체시험연구

In vitro Alternative Study of Phototoxitiy by Neutral Red Uptake Assay Using Human Fibroblast

  • 이종권 (식품의약품안전청 국립독성연구소 독성부) ;
  • 이은희 (식품의약품안전청 국립독성연구소 독성부) ;
  • 김형수 (식품의약품안전청 국립독성연구소 독성부) ;
  • 홍진태 (식품의약품안전청 국립독성연구소 독성부) ;
  • 류승렬 (식품의약품안전청 국립독성연구소 독성부) ;
  • 박기숙 (식품의약품안전청 국립독성연구소 독성부) ;
  • 김대병 (식품의약품안전청 국립독성연구소 독성부) ;
  • 김부영 (식품의약품안전청 국립독성연구소 독성부) ;
  • 조대현 (식품의약품안전청 국립독성연구소 독성부)
  • 발행 : 2000.03.01
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초록

This study was conducted to assess a possible alternative method as replacements for in vivo test. The human fibroblasts were exposed to several photoxic chemicals (promethazine, neutral red, chlortetracyclone, amiodatone, bithional, 8-methyooxypsorale) and non-phototoxi substance, ammonium laureth sulfate and irradiatied with 5 J/$cm^2$ of UVA (3320~420nm). The cell viability was measured by NRU (neutral red uptake) assay. The photoxic potential of test chemicals in the NRU PT (phototoxicity test) was assessed by determining the PIF (photoirritancy Factor) by using a cut-off value of 5. The NRU PT responses of most chemicals showed a close agreement with in vivo response except bithinol. There was a relatively good agreement between in vitro NRU assay and in vivo data. These results suggest that NRU assay using fibroblast could be used to predict the phototoxicity.

키워드

참고문헌

  1. ATLA v.25 Current status and future developments of databases on alternative methods Annette, J.;Margot, D.O.;Krys, B.;Barbara, G.;David, C.A.
  2. Toxicol. Lett. v.24 Toxicity determined in vitro by Morphological alterations and neutral red absorption Borenfreund, E.;Puerner, J.A.
  3. J. Toxico. Pub Health v.14 no.1 Comparison MTT assay with neutral red uptake assay in measuring phototoxicity in vitro Chung, S.T.;Han, H.M.;Kim, J.H.;Kim, P.S.;Kang, S.K.;Kim, H.S.;Kim, J.I.
  4. ATLA v.27 The use of human keratinocytes in the EU/COLIPA international in vitro phototoxicity test validation study and the ECVAM/COLIPA study on UV filter chemicals Clothier, R.;Willshaw, A.;Cox, H.;Garle, M.;Bowler, H.;Combes, R.
  5. J. Phram. Exp. Ther. v.83 Methods for study of irritation and toxicity of substances applied topically to the skin and mucous membranes Draize, J.H.;Woodard, G.;Calvery, H.O.
  6. ATLA v.25 A general measure of in vitro phototoxicity derived from pairs of dose-response curves and its use for predicting the in vivo phototoxicity of chemicals Holzhutter, H.G.
  7. J. Toxicol. Pub Health v.15 no.3 Alternative methods in phototoxicity Kim, Y.O.;Chung, H.J.;Chung, S.T.;Cho, D.H.
  8. J. Invest Dermatol. v.98 Quantitative in vitro assessment of phototoxicity by a fibroblast-neutral red assay Lasarow, R.M.;Isseroff, R.;Gomez, E.C.
  9. J. Toxicol. Pub Health v.14 no.3 In vitro skin irritation test of anti-inflmmatory drugs Lee, J.K.;Kim, D.B.;Lee, E.H.;Lee, S.H.;Choi, K.H.;Kim, Y.J.;Kim, P.Y.
  10. J. Toxicol. Pub Health v.15 no.2 Comparison of cultured fibroblasts and HaCaT cells by Alamar Blue assay to predict skin irritation potential of surfactants Lee, J.K.;Kim, D.B.;Lee, E.H.;Lee, H.;Kim, P.Y.
  11. J. Toxicol. Pub Health v.15 no.2 In vitro skin irritation test of humectants with human skin fibroblasts Lee, J.K.;Kim, D.B.;Lee, E.H.;Ryu, S.R.;Kim, Y.J.;An, S.S.;Lee, B.M.;Lee, Y.S.;Kim, P.Y.
  12. J. Pharmacol. Exp. Ther. v.96 A simplified method of evaluating dose effect experiments Liichfield, J.T.;Wilcoxon, F.
  13. Draft proposal for a new guideline-Acute dermal photoirritation screening test OECD Gudeline for testing of chemicals
  14. ATLA v.27 In vitro alternatives and phototoxicity testing. Ⅰ. Evaluation of in vitro phototoxicity assays Okamoto, Y.;Ryu, A.;Ohkoshi, K.
  15. Toxin in vitro v.8 no.4 EEC/COLIPA project on in vitro phototoxicity testing: First results obtained with a balb/c 3T3 cell phototoxicity assay Spielmann, H.;Balls, M.;Brand, M.;Doring, B.;Holzhutter, H.G.;Kalweit, S.;Klecak, G.;Eplattenier, H.L.;Liebsch, M.;Lovell, W.W.;Maurer, T.;Moldenhauer, F.;Moore, L.;Pape, W.J.W.;Pfannenbecker, U.;Potthast, J.M.;O. De. Silva O.D.;Casto, M.;Sladowski, D.;Steiling, W.;Willshaw, A.
  16. ATLA v.22 In vitro phototoxicity testing. The report and recommendations of ECVAM workshop 2 Spielmann, H.;Lovell, W.W.;Holze, E.;Johnson, B.E.;Maurer, T.;Miranda, M.A.;Pape, W.J.W.;Sapora, O.;Sladowski, D.
  17. Curr. Probl. Dermatol. v.23 EEC/COLIPA in vitro photoirritancy program: Results of the first stae of validation Spielmann, H.;Liebsch, M.;Pape, W.J.W.;Balls, M.;Dupuis, J.;Klecak, G.;Lovell, W.W.;Maurer, T.;De Silva O.;Steiling, W.
  18. ATLA v.26 A study on UV filter chemicals from annex VII of European union directive 76/768/EEC. in the in vitro 3T3 NRU phototoxicity test Spielmann, H.;Balls, M.;Dupuis, J.;Pape, W.J.W.;de Silva O.;Holzhutter, H.;Gerberick, F.;Liebsch, M.;Lovell, W.W.;Pfannenbecker, U.
  19. Toxic in vitro v.12 The international EU/COLIPA In vitro phototoxicity validation study: Results of phase Ⅱ (blind trial). Part Ⅰ: The 3T3 NRU Phototoxicity Spielmann, H.;Balls, M.;Dupuis, J.;Pape, W.J.W.;Pechovitch, G.;De Seilva O.;Holzhutter, H.;Clothicr, R.;Desolle, P.;Gerberick, F.;Licbsch, M.;Lovell, W.W.;Mauerer, T.;Pfannenbecker, U.;Potthast, J.M.;Casto, M.;Sladowski, D.;Steiling, W.;Brantom, P.