Toxicological Research

  • 제16권1호
  • /
  • Pages.63-66
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  • 2000
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  • 1976-8257(pISSN)
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  • 2234-2753(eISSN)
한국독성학회 (Korean Society of Toxicology & Korea Environmental Mutagen Society)
권호 표지

Acute Ethanol Reduces Calcium Signaling Elicited by K+ Depolarization in Cultured Cerebellar Granule Neurons

  • 발행 : 2000.03.01
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초록

The effects of acute ethanol on the high K+ induced $Ca^{2+}}$ signals were examined from primary cultures of cerebellar granule neurons. $Ca^{2+}}$ signals were measured with Calcium Green-1 based microscopic video imaging. Because $Ca^{2+}}$ signal was low in most of granule neurons without stimuli, high KCI was used for depolarization. In most case, acute exposure to ethanol reduced the peak amplitude of the $Ca^{2+}}$ signals, induced by high K+, even though low concentration of ethanol(2~10mM) was used and the effects lasted more than 30min. In was also possible to see differences of ethanol inhibition, i.e. the temporal pattern of $Ca^{2+}}$ signal reductions and the strength of inhibition of $Ca^{2+}}$ signals in cerebellar granule neurons. These results indicate that low concentration of ethanol has diverse actions on the $Ca^{2+}}$ signals in cerebellar granule neurons.

키워드

참고문헌

  1. Alcohol v.14 Developmental changes in the inhibitory actions of ethanol on glutamate-induced translocation of protein kinase C in cerebellar granule neurons Barrios, M.;Liljequist, S.
  2. Alcohol v.4 Electrophysiological action of ethanol at the cellular level Bloom, F.E.;Siggins G.R.
  3. Alcohol v.10 Effective stabilization of ethanol levels in multiple-well tissue cultrue plates Borgs, P.;Way, D.L.;Witte, M.H.;Witte, C.L.
  4. Brain Res. v.499 Ethanol inhibits NMDA-induced increases in free intracellular Ca²+ in dissociated brain cells Dildy, J.E.;Leslie, S.W.
  5. Alcohol Clin. Exp. Res. v.22 Effects of moderate alcohol consumption on the central nervous system Eckardt, M.J.;File, S.E.;Gessa, G.L.;Grant, K.A.;Guerri, C.;Hoffman, P.L.;Kalant, H.;Koob, G.F.;Li, T.K.;Tabakoff, B.
  6. Brain Res. v.416 Acute ethanol alters the firing pattern and glutamate response of cerebellar Purkinje neurons in culture Franklin, C.L.;Gruol, D.L.
  7. Brain Res. v.469 Morphological and physiological properties of rat cerebellar neurons in matue and developing cultures Gruol, D.L.;Crimi, C.P.
  8. Brain. v.673 Calcium signals elicited by quisqualate in cultured Purkinje neurons show developmental changes in sensitivity to acute alcohol Gruol, D.L.;Curry, J.G.
  9. Brain Res. v.773 Acute ethanol alters calcium signals elicited by glutamate receptor agonists and $K^+$ depolarization in cultured cerebellar Purkinje neurons Gruol, D.L.;Parsons, K.L.;DiJulio, N.
  10. J. Neurochem v.52 N-methyl-D-aspartate receptors and ethanol: inhibiton of calcium flux and cyclic GMP production Hoffman, P.L.;Rabe, C.S.;Moses, F.;Tabakoff, B.
  11. Eur. J. Pharmacol. v.309 N-Methyl-D-aspartate-induced enhancement of activator protein-1 DNA binding activity is blocked by ethanol in cerebellar granule cells Hou, Y.N.;Cebers, G.;Cebere, A.;Terenius, L.;Liljequist, S.
  12. Brain Res. v.701 Acute alcohol blocks neurosterold modulation of synaptic transmission and long-term potentiation of synaptic transmission and long-term potentiation in the rat hippocampal slice Randall, R.D.;Lee, S.Y.;Meyer, J.H.;Wittenberg, G.F.;Gruol, D.L.
  13. Exp. Neurol. v.55 Direct effects of ethanol on central nervous system cultures: an electrophysiological and morphological study Seil, F.J.;Leiman, A.L.;Herman, M.M.;Fisk, R.A.
  14. Pharmacol. Biochem. Behau v.14 Ethanol-induced depressions in cerebellar and hippocampal neurons of mice selectively bred for differences in ethanol sensitivity: an electrophysiological study Sorenson, S.;Dunwiddie, T.;McClearn, G.;Freedman, R.;Hoffer, B.
  15. Brain Res. v.569 Acute alcohol alters the excitability of cerebellar Purkinje neurons and hippocampal neurons in culture Urrutia, A.;Gruol, D.L.