Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
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Effect of the bee venom aqua-acupuncture on the neuronal activities of serotonergic system in brainstem

봉독약침자극(蜂毒藥鍼刺戟)이 뇌간(腦幹) 신경세포(神經細胞)와 Serotonin성(性) 신경세포(神經細胞)의 활성변화(活性變化)에 미치는 영향

  • Kim, Hye-Nam (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Koh, Hyung-Kyun (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Park, Dong-Suk (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Kang, Sung-Keel (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Kim, Yong-Suk (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Choi, Yong-Tae (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University)
  • Published : 2000.06.20
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Abstract

This study was designed to evaluate the effect of the bee venom(BV) aqua-acupuncture on the neuronal activities of serotonergic(5-HT) system in the brainstem. After the BV aqua-acupuncture was applied on Chok-Samni(ST36) and the gluteal part(Blank locus) in rats, the number of Fos immunoreactive neurons was counted by using computerized image analyzing system. Also, the number of colocalization between 5-HT containing neurons Fos immunoreactive neurons were analyzed by using the double immunohistochemical technique. The results of the experiments were summarized as follows : 1. In almost every neucli, the Chok-Samni group and Blank locus group showed more increase in the number of Fos immunoreactive neurons than the control group. Especially, in Arc, DR, LC, RMg, Gi, PAG Rost and PAG LV, the Chok-Samni group showed more significant increase than the control group. Also, in PAG LV Mid and Arc, Chok-Samni group showed more significant increase than the Blank locus group. 2. In DR and PAG LV Mid, Chok-Samni group and the Blank locus group showed more significant increase in the number of colocalization between 5-HT containing neurons and Fos immunoreactive neurons than the control group after the BV aqua- acupuncture. Also, the Chok-Samni group showed more significant increase than the Blank locus group. Consequently, the BV aqua-acupuncture increased more potent the number of Fos immunoreactive neurons and the activity of serotonergic neurons. Furthermore, the BV aqua-acupuncture was more effective on Chok-Samni than Blank locus group. These results indicate that the BV aqua-acupuncture is very effective therapy to control pain. The therapeutic effect of BV aqua-acupunture may associated with the endogenous modulatory system such as serotonin Those data from the study can be applied to establish the effective treatment of the BV for pain control in the clinical field.

Keywords

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