Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

Molecular Biological Study of Anti-cancer Effects of Bee Venom on Human Melanoma Cell

약침용봉독액(藥鍼用蜂毒液)이 흑색종세포(黑色腫細胞)에 미치는 항암효과(抗癌效果)에 대(對)한 분자생물학적(分子生物學的) 연구(硏究)

  • Park, Chan-Yol (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Nam, Sang-Soo (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Kim, Chang-Hwan (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Lee, Jae-Dong (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Kang, Sung-Keel (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Lee, Yun-Ho (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University) ;
  • Ahn, Byoung-Choul (Dept. of Acupuncture & Moxibustion Oriental Medical College, Kyung Hee University)
  • 박찬렬 (경희대학교 한의과대학 침구학교실) ;
  • 남상수 (경희대학교 한의과대학 침구학교실) ;
  • 김창환 (경희대학교 한의과대학 침구학교실) ;
  • 이재동 (경희대학교 한의과대학 침구학교실) ;
  • 강성길 (경희대학교 한의과대학 침구학교실) ;
  • 이윤호 (경희대학교 한의과대학 침구학교실) ;
  • 안병철 (경희대학교 한의과대학 침구학교실)
  • Published : 2000.06.20
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Abstract

To study anti-cancer effect and molecular biological mechanism of bee venom for aqua-acupuncture, the effects of bee venom on cell viability, apoptosis, and cell cycle were analyzed using MTT assay, tryphan blue assay, [3H]thymidine release assay, flow cytometric analysis, activity of caspase-3 protease activity assay, and immunocytometric analysis of PCNA. To explore whether anti-cancer effects of bee venom are associated with the transcriptional control of gene expression, quantitative RT-PCR analysis of apoptosis- and cell cycle-related genes was performed. The obtained results are summarized as follows: 1. The MTT assay demonstrated that cell viability was decreased by bee venom in a dose-dependant manner. 2. Significant induction of apoptosis was identified using tryphan blue assay, [$^3H$]thymidine release assay, and flow cytometric analysis of sub $G_1$ fraction. 3. In analysis of caspase-3 protease activity, the activity had increased significantly, in a dose-dependant manner. 4. Quantitative RT-PCR analysis of the apoptosis-related genes showed that Bcl-2 and $Bcl-X_L$ were down-regulated whereas Bax was up-regulated by bee venom treatment. 5. In flow cytometric analysis of cell cycle and immunocytometric analysis of PCNA expression, cell numbers of $G_1$ phase was increased by a dose-dependant manner. 6. In quantitative RT-PCR analysis of the cell cycle-related genes, p21, p27, and p57 were increased, while Cyclin D1, CDK4, c-Myc, c-Fos, and Histone H3 were decreased. In contrast, there were no remarkable changes in expression levels of CDC2 and c-Jun.

Keywords

References

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