Journal of Acupuncture Research

Korean Acupuncture & Moxibustion Medicine Society (대한침구의학회)
권호 표지

The antimutagenic effect and genetic safety of Buthus martensi Karsch aqua-acupuncture solution (BMKAS)

전갈(全蝎) 약침액(藥鍼液)의 항돌연변이(抗突然變異) 및 항암(抗癌) 효과(效果)

  • Kim, So-Houng (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong Guk University) ;
  • Kim, Kap-Sung (Department of Acupuncture & Moxibustion, College of Oriental Medicine, Dong Guk University)
  • 김소형 (동국대학교 한의과대학 침구학교실) ;
  • 김갑성 (동국대학교 한의과대학 침구학교실)
  • Received : 2000.08.10
  • Accepted : 2000.08.26
  • Published : 2000.09.20
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Abstract

Objective : The aim of this study is to determine the antimutagenic effect and genetic safety of Buthus martensi Karsch aqua-acupuncture solution(BMKAS) against various chemical carcinogens. Method : Ames(Salmonella typhimurium) test and Rec assay(Bacillus subtilis) were used as indicators for DNA damage and antimutagenesis. Furthermore, the levels of umu operon expression by measuring the ${\beta}$-galactosidase activity wete monitored with the SOS umu test using S. typhimurium 1535 containing plasmid pSK1002. And the host-mediated assay was used to investigate the mutagenicity and antimutagenicity of BMKAS inducing various chemical carcinogens after the activation with in vivo metabolic systems. Results : From the results, BMKAS did not atfect DNA of S. typhimurium and B. subtilis strains and showed no mutagenicity at the all concentrations of tested solution. Furthermore BMKAS dose-dependently protected the mutagenecity by AF-2, 2-AA and B[a]P. These phenomena was also similar to that after metabolic activation of BMKAS in in vivo system. Conclusion : These results suggested that BMKAS did not show the mutagenicity and protected the mutagenesis against various chemical carcinogens by four different methods used in this study.

Keywords

References

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