Effect of Pasteurized Substrates in Various Condition on the Mycelial Growth of Oyster Mushroom, Pleurotus spp.

볏짚배지의 살균조건이 느타리버섯균의 균사생장에 미치는 영향

  • Jhune, Chang-Sung (Applied Microbiology Division, Department of Bio-Resources, National Institute of Agricultural Sciences and Technology, R.D.A.) ;
  • Shin, Dong-Hun (Kapyung-Gun Agricultural Technology Center) ;
  • Park, Jeong-Sik (Applied Microbiology Division, Department of Bio-Resources, National Institute of Agricultural Sciences and Technology, R.D.A.) ;
  • Oh, Se-Jong (Applied Microbiology Division, Department of Bio-Resources, National Institute of Agricultural Sciences and Technology, R.D.A.)
  • 전창성 (농촌진흥청 농업과학기술원 응용미생물과) ;
  • 신동훈 (농촌진흥청 가평군 농업기술센터) ;
  • 박정식 (농촌진흥청 농업과학기술원 응용미생물과) ;
  • 오세종 (농촌진흥청 농업과학기술원 응용미생물과)
  • Published : 2000.10.30

Abstract

Various conditions for mushroom were investigated to study their effects on the mycelial growth of oyster mushroom and pathogens. Our results show that the optimal pasteurization temperatures for mycelial growth and pathogens are $60^{\circ}C\;and\;121^{\circ}C$ respectively. Both were then decreased by heating over $60^{\circ}C$ and getting down temperature under $121^{\circ}C$, respectively. In the experiment for pasteurization time, it was showed that both 8 and 12 hours pasteurization were greater than 24 hours treatment for the mycelial growth. In contrast, we didn't find any particular effect by different soaking time of substrates. The pH In substrates was leveled well by pasteurization and after-fermentation even though the acidity was lowered by extending the soaking time. Our results also show that the mycelial growth is greatest at $25{\sim}30^{\circ}C$ regardless of pasteurization time for substrates, Through the experiment with paddy straw as a substrate, it was showed that two time pasteurization was greater than one time for mycelium growth and pro-fermentation of paddy straw before pasteurization have resulted in worth mycelial growth compared with that of normal condition.

느타리버섯 재배용 볏짚배지의 살균과 관련하여 균사생장에 미치는 영향을 실내에서 시험한 결과, 느타리버섯의 균사생장은 $60^{\circ}C$에서 살균한 것이 가장 우수하였고 살균온도가 높아질수록 감소되었으며, 병원균의 생장은 $121^{\circ}C$에서 가장 양호하고 온도가 낮을수록 감소되었다. 볏짚배지의 살균시간은 8, 12시간에서 느타리버섯의 균사생장이 가장 양호하였고 24시간 처리에서는 오히려 균사생장이 감소되었다. 살균시간과 침수시간에 따른 배지 pH의 변화는 침수시간이 길어지면 산도가 낮아지고 살균과 후발효후 종균접종시에는 전반적으로 균형을 이루었다. 느타리버섯 볏짚배지의 살균시간에 상관없이 균사생장정도는 $25{\sim}30^{\circ}C$에서 가장 양호하였다. 느타리버섯의 볏짚배지는 2회 살균보다는 1회 살균시 균사생장이 양호하였다. 미리 균상 내에서 전발효를 하는 경우는 오히려 정상적인 것보다는 균사생장이 불량하였다.

Keywords

References

  1. The Biology and Technology of the Cultivated Mushroom Flegg, P.B.;Spencer, D.M.;Wood, D.A.
  2. 최신버섯재배기술 차동열;유창현;김광포
  3. 느타리버섯 병해 발생현황조사. 농촌진흥청 농업기술연구소 시험보고서(생물부편) 1998 전창성
  4. 느타리버섯 볏짚 조제방법 개선에 관한 시험. 농촌진흥청 농업기술연구소 시험보고서 1979 고승주;박용환;차동열
  5. M.G.A. Bull. v.271 Pleurotus ostreatus has great possibilities Lelly, J.
  6. 느타리버섯 볏짚재배의 조제 방법 개선에 관한 시험. 농촌진흥청 농업기술연구소 시험보고서(생물부편) 1975 박용환;고승주
  7. 느타리버섯 볏짚재배의 조제 방법 개선에 관한 시험. 농촌진흥청 농업기술연구소 시험보고서(생물부편) 박용환;고승주;장현세;김영배
  8. Scientia Horticulturae v.20 The significance of thermophilic fungi in mushroom compost preparation Ross, R.C.;Harris, P.J.
  9. Science Horticulturae v.17 Some factors involved in phase II of mushroom compost preparation Ross, R.C.;Harris, P.J.
  10. Mushroom Sci. v.8 Micro-organisms inhabiting mushroom compost during fermentation Stanek, M.
  11. Horti, Abs v.43 Ein never pilz (Pleurotus ostreatus) Der Champignon 12: 5-11 Schmaus, F.
  12. Mushroom Sci. v.9 The ecology and industrial production of Pleurotus ostreatus, P. florida, P. cornucopiae and P. eryngii Zadrazil, F.