The Korean Journal of Mycology (한국균학회지)

The Korean Society of Mycology (한국균학회)
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The Characteristics of Thermophilic Fungi in Relation to Growth-Promoting Effect on the Mycelium of Pleurotus ostreatus

Pleurotus ostreatus 균사의 생장 촉진 효과를 나타내는 고온성 곰팡이의 특징

  • Lee, Ho-Yong (Department of Biological Science, Sang Ji University) ;
  • Shin, Chang-Yup (Department of Biological Science, Sang Ji University) ;
  • Kim, Jun-Ho (Department of Chemistry, Sang Ji University) ;
  • Kim, Won-Rok (Institute of Biological Science, Sang Ji University) ;
  • Lee, Young-Keun (Radiation Application Team, Korea Atomic Energy Research Institute) ;
  • Chang, Hwa-Hyoung (Radiation Application Team, Korea Atomic Energy Research Institute) ;
  • Song, In-Geun (Radiation Application Team, Korea Atomic Energy Research Institute) ;
  • Hyun, Soung-Hee (Department of Premedicine, Eulji University School of Medicine) ;
  • Min, Bong-Hee (Department of Biology, Taegu University)
  • 이호용 (상지대학교 생명과학과) ;
  • 신창엽 (상지대학교 생명과학과) ;
  • 김준호 (상지대학교 화학과) ;
  • 김원록 (상지대학교 생명과학연구소) ;
  • 이영근 (한국원자력연구소 방사성응용연구팀) ;
  • 장화형 (한국원자력연구소 방사성응용연구팀) ;
  • 송인근 (한국원자력연구소 방사성응용연구팀) ;
  • 현성희 (을지의과대학교 의예과) ;
  • 민봉희 (대구대학교 생물학과)
  • Published : 2000.10.30
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Abstract

The mycelial growth of Pleurotus ostreatus in compost is strongly stimulated by solid-state fermentation with thermophilic fungi which were isolated from oyster mushroom compost. The biochemical characteristics of these thermophilic fungi were investigated. Cellulase and ligninase activities were not detected by clear zone effect on CMC and lignin media. All of thermophilic fungi grew well with high mycelial density on xylan media and the growing rate of Sepedonium sp. S-2 observed very high. In results of MUF-test, extracellular enzyme activity of Sepedonium sp. S-2, and S-5 measured very high. On the compost after high temperature fermentation with Sepedonium sp. S-2 and S-5, the mycelial growing rate of Pleurotus ostreatus was increased about 50% and it also showed the inhibiting effect on mycelial growth of Trichoderma sp. SJG-51. Isolated thermophilic fungi, Sepedonium sp. S-2 and S-5 were expected as very useful organism for making oyster mushroom compost.

느타리버섯의 균사 생장을 촉진시키고 병원성 균류의 생장을 억제하므로 느타리버섯의 생산을 증가시키는 고온성 미생물을 분리하고 그 원인을 생화학적으로 조사하였다. 느타리버섯 배지로부터 분리한 7종의 고온성 곰팡이에서 모두 lignin 분해 활성 능은 확인되지 않았고, xylanase 분해능은 모든 분리 균에서 확인되었으며, 특히 H. grisea var. thermoidea와 Sepedonium sp. S-2 분리균류에서 높은 활성을 나타내었다. Cellulose 배지에서 균사 생장은 나타났으나 그 분해는 확인 할 수 없는 반면 MUF-test로 확인한 세포외 분비 cellulase의 활성도는 Sepedunium sp. S-2와 S-5에서 가장 높은 활성을 나타내었다. 이들 두 균류는 느타리버섯 생장을 50%증가시켰으며, 푸른곰팡이병 원인균인 Trichoderma sp. SJG-51에 대한 생장억제효과도 나타내었다 이상의 결과로 보아 고온성 곰팡이 Sepedonium sp. 일부는 느타리버섯 재배 시 배지의 물성변화를 일으키는 효소를 분비하여 버섯 생장률 증가 및 유해균류의 생장을 억제하였다 따라서 느타리버섯 배지 제조에 있어 이들 고온성 곰팡이들이 매우 유용할 것으로 판단된다.

Keywords

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